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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Overview
Product name
Anti-VDAC/Porin antibody
See all VDAC/Porin products (9) ...
Description
Rabbit polyclonal to VDAC/Porin
Specificity
ab28777 recognises Outer mitochondrial membrane protein porin (VDAC1).
Tested applications
IHC-P, WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Cow, Dog, Pig, Zebrafish
Immunogen
A region within synthetic peptide: SAKVNNSSLI GLGYTQTLKP GIKLTLSALL DGKNVNAGGH KLGLGLEFQA, corresponding to C terminal amino acids 234-283 of Human VDAC/Porin
SAKVNNSSLI GLGYTQTLKP GIKLTLSALL DGKNVNAGGH KLGLGLEFQA
Positive control
HepG2 cell lysate. Human liver tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Cellular metabolic process
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Mitochondrial
Neuroscience >> Neurotransmission >> Receptors / Channels >> More Ion Channels
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 4.0 - 8.0 µg/ml.
WB: Use a concentration of 1.25 µg/mlPredicted molecular weight: 31 kDa.
ELISA
ELISA: 1/312500ELISA: 1/312500
Target
Function
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
Tissue specificity
Heart, liver and skeletal muscle.
Sequence similarities
Belongs to the eukaryotic mitochondrial porin family.
Domain
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
Cellular localization
Mitochondrion outer membrane. Cell membrane.
Target information above from: UniProt accessionP21796
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hVDAC1 antibody
- MSA08 antibody
- Outer mitochondrial membrane protein porin 1 antibody
see all
Database links
- Entrez Gene: 282119 Cow
- Entrez Gene: 7416 Human
- Entrez Gene: 22333 Mouse
- Entrez Gene: 397010 Pig
- Entrez Gene: 83529 Rat
- Entrez Gene: 334582 Zebrafish
- Omim: 604492 Human
- SwissProt: P45879 Cow
see all
Anti-VDAC/Porin antibody images:
Western blot - VDAC/Porin antibody (ab28777)
Anti-VDAC/Porin antibody (ab28777) at 1.25 µg/ml + HepG2 cell lysate
Secondary
HRP conjugated anti-Rabbit IgG
Predicted band size : 31 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%
Immunohistochemistry (Paraffin-embedded sections) - VDAC/Porin antibody (ab28777)
ab28777, at a concentration of 6.0µg/ml, staining human VDAC1 in (liver) by Immunohistochemistry, Paraffin embedded tissue. Arrows indicate positively labelled cells (hepatocytes). Magnification 400X.
References for Anti-VDAC/Porin antibody (ab28777)
This product has been referenced in:
- Chen Set al. Reduced expression of lamin A/C results in modified cell signaling and metabolism coupled with changes in expression of structural proteins. J Proteome Res 8:5196-211 (2009).Read more (PubMed: 19775189) »
See 1 publication for this product
Publishing research using ab28777? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"