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Overview

  • Product nameAnti-VDAC/Porin antibodySee all VDAC/Porin primary antibodies ...
  • Description
    Rabbit polyclonal to VDAC/Porin
  • Specificityab28777 recognises Outer mitochondrial membrane protein porin (VDAC1).
  • Tested applicationsIHC-P, WB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Pig, Zebrafish
  • Immunogen

    A region within synthetic peptide: SAKVNNSSLI GLGYTQTLKP GIKLTLSALL DGKNVNAGGH KLGLGLEFQA, corresponding to C terminal amino acids 234-283 of Human VDAC/Porin

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHepG2 cell lysate. Human liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab28777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: Use at an assay dependent concentration.
WB WB: Use a concentration of 1.25 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA ELISA: 1/312500.

Target

  • Alternative names
      N2441 antibody OMP2 antibody POR1 antibody
      hVDAC1 antibodyOuter mitochondrial membrane protein porin 1 antibodyPlasmalemmal porin antibodyPorin 31HL antibodyPorin 31HM antibodyVDAC antibodyVDAC-1 antibodyVdac1 antibodyVDAC1_HUMAN antibodyVoltage dependent anion channel 1 antibodyVoltage dependent anion selective channel protein 1 antibodyVoltage-dependent anion-selective channel protein 1 antibodyYNL055C antibodyYNL2441C antibody
    see all

Anti-VDAC/Porin antibody images

  • Western blot - VDAC/Porin antibody (ab28777)
    Western blot - VDAC/Porin antibody (ab28777)
    Anti-VDAC/Porin antibody (ab28777) at 1.25 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size : 31 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Paraffin-embedded sections) - VDAC/Porin antibody (ab28777)
    Immunohistochemistry (Paraffin-embedded sections) - VDAC/Porin antibody (ab28777)
    ab28777, at a concentration of 6.0µg/ml, staining human VDAC1 in (liver) by Immunohistochemistry, Paraffin embedded tissue. Arrows indicate positively labelled cells (hepatocytes). Magnification 400X.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-VDAC/Porin antibody(ab28777)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-VDAC/Porin antibody(ab28777)
    Immunohistochemistry of Human Liver cell lysate tissue at an antibody concentration of 4-8µg/ml using anti-VDAC1 antibody (ab28777)

References for Anti-VDAC/Porin antibody (ab28777)

This product has been referenced in:
  • Arduíno DM  et al. Mitochondrial metabolism in Parkinson's disease impairs quality control autophagy by hampering microtubule-dependent traffic. Hum Mol Genet 21:4680-702 (2012). WB ; Human . Read more (PubMed: 22843496) »
  • Chen S  et al. Reduced expression of lamin A/C results in modified cell signaling and metabolism coupled with changes in expression of structural proteins. J Proteome Res 8:5196-211 (2009). Read more (PubMed: 19775189) »

See all 2 Publications for this product

Publishing research using ab28777 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab28777 Submit a Question for ab28777

Displaying 1 - 3 of 3 results for Abreviews and Q&A

10
Votes: Highest First

Thanks for your email,
if you look at the protein of interest(VDAC1) in data base, the mw of the protein is 31-35 kd and not more, this is what exactly we see in the blots with a good anti vdac1 from abcam (cat no 15895),and normally if we do see ban...

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Thank you for contacting Abcam.

I am sorry about the issues you have been having with ab28777 in western blot and I do understand why you are concerned about the results you have been receiving with the antibody. As requested I am sending you a ...

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Thanks for your email,
actually I have 2 anti-VDAC1 from abcam that doesn't work for us includes cat# **attachment you can find all the answers to your questions,hope it helps
looking forward to hearing from you.

Thank you for your reply and for providing that extra information.

From the image that you have submitted, it does look as though the antibody ab28777 is detecting a band at about 50kDa, rather than 32kDa. But the band it is detecting is pretty ...

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In may 2012 we purchased Anti-VDAC1 antibody cat # 28777 ,and so far we tried with this and it didn't work for us

we would like to know if it is possible that we get another anti- vdac1 from your company. Can we have a batch of

anti-...

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Thank you for contacting Abcam.

I am sorry about the issues you are having with ab28777. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot onhumansamples . If we cannot resolve the issue you are...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"