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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Anti-VDAC/Porin antibody (ab34726)
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab34726? Please let us know so that we can cite the reference in this datasheet
ab34726 has been referenced in 2 publications.
- Machado NG et al. Sub-chronic administration of doxorubicin to Wistar rats results in oxidative stress and unaltered apoptotic signaling in the lung. Chem Biol Interact 188:478-86 (2010). WB; Rat. PubMed: 20932959
- Sun F et al. Mitochondrial accumulation of polyubiquitinated proteins and differential regulation of apoptosis by polyubiquitination sites Lys-48 and -63. J Cell Mol Med : (2009). WB; Rat. PubMed: 19432818
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-VDAC/Porin antibody(ab34726)
IHC image of ab34726 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34726, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - VDAC/Porin antibody (ab34726)
ab34726 at a 1/100 dilution staining VDAC/Porin in COS7 cells by Immunocytochemistry/ Immunofluorescence.The antibody does not work with a standard fixation for ICC/IF as it does not recognize the non-denatured protein. However, applying a fixation for hidden antigens (modified from Peränen J, Rikkonen M, Kääriäinen L. A method for exposing hidden antigenic sites in paraformaldehyde-fixed cultured cells, applied to initially unreactive antibodies. J Histochem Cytochem. 1993 Mar41(3):447-54) results in very good recognition of VDAC/porin. Denaturing is achieved by Guanidine hydrochloride in 50 mM Tris-HCL pH 7.5 after fixation (3% pFA, 0.05% GA) and permeabilization (0.2% TX-100). The secondary used was a TRITC conjugated donkey anti-rabbit used at a 1/100 dilution.
Image courtesy of M Schrader by Abreview.
VDAC/Porin antibody for WB in Rat (34726)
VDAC/Porin antibody for ICC in African Green Monkey (34726)
VDAC/Porin antibody for Western blot in Human (34726)
VDAC/Porin antibody for Western blot in Pig (34726)
VDAC/Porin antibody for ICC/IF in African Green Monkey (34726)
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