Anti-VDAC/Porin antibody (ab34726)

Dear Technical,



Good day! We have a customer who has placed an order for the above antibody but it is in back order and will not be available before March. Our customer is wondering if there is any alternative antibodies that they can use instead, as they need this before the middle of Feb.



I was wondering if you could advise me about any alternative antibody that can be used.



I hope to hear from you soon.



Kind regards,

ANSWER:

Thank you for your enquiry.
Currently, this antibody (ab34726) is out of stock. However, the good news is that we have some alternative products against the same target protein (VDAC/Porin). I do not know in which species/application your customer would like to use the other product so please advice her/him to read the products datasheets carefully to see which one would be suitable.
- ab110326: http://www.abcam.com/vdac-porin-antibody-16g9e6bc4-ab110326.html
- ab61273: http://www.abcam.com/vdac-porin-antibody-mitochondrial-marker-ab61273.html
- ab96140: http://www.abcam.com/vdac-porin-antibody-16g9e6bc4-ab110326.html
I hope this helps and if I can assist further, please do not hesitate to contact me.

Our customer would like to use ab14734 for IHC-P, staining of human cancer sections (from different origins) and healthy human sections. Kindly advise if this specific antibody (ab14734) will help to distinguish between healthy and cancer tissue? In addition to that, please explain the difference between  Anti-VDAC1 / Porin antibodies with the addition of "Mitochondrial Loading Control" after the name of the antibody (like ab14734) and Anti-VDAC1 / Porin antibodies that lack this description (ab34726, for ex). Thanks in advance for your assistance.    

ANSWER:

Thank you for your inquiry.

VDAC1 is a mitochondrial protein and I believe its level of expression is pretty ubiquitous in all tissues, cancerous and normal.

According to the Human Protein Atlas, VDAC1 is expressed in most normal and cancer tissues tested.

http://www.proteinatlas.org/ENSG00000213585/normal

http://www.proteinatlas.org/ENSG00000213585/cancer

All anti-VDAC1/Porin antibodies in our catalogue will stain the same mitochondrial protein. The ones named "Mitochondrial Loading Control" have typically been cited in papers to have been used as such. That is really the only main difference in the name.

I hope this information helps. Please contact us with any other questions.

What percentage of Guanidine HCl should be used to prepare fixed cells for ICC and how long should they be treated for?

ANSWER:

Thank you for your inquiry.

Cells should be permeabilized with Triton X-100 and then incubated in 6 N Guanidine HCl in 50mM Tris-HCl, pH 7.5 for 10 minutes at room temperature.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.