Anti-VDAC/Porin antibody (ab96140)

Overview

• Product nameAnti-VDAC/Porin antibodySee all VDAC/Porin primary antibodies ...
• Description
  Rabbit polyclonal to VDAC/Porin
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Pig
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human VDAC/Porin.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab109894.)

• Positive controlThis antibody gave a positive signal in Human skeletal muscle tissue lysate as well as the following whole cell lysates: HeLa; HepG2; HEK293.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Cancer
  • Cancer Metabolism
  • Cellular metabolic process

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Signal Transduction
  • Metabolism
  • Plasma Membrane
  • Channels

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

Applications

Target

• FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
• Tissue specificityHeart, liver and skeletal muscle.
• Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
• DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
• Cellular localizationMitochondrion outer membrane. Cell membrane.

Target information above from: UniProt accession P21796 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 282119 Cow
  • Entrez Gene: 7416 Human
  • Entrez Gene: 7416 Human
  • Entrez Gene: 7416 Human
  • Entrez Gene: 7416 Human
  • Entrez Gene: 22333 Mouse
  • Entrez Gene: 397010 Pig
  • Entrez Gene: 100008751 Rabbit

  • Entrez Gene: 83529 Rat
  • Omim: 604492 Human
  • SwissProt: P45879 Cow
  • SwissProt: P21796 Human
  • SwissProt: P21796 Human
  • SwissProt: P21796 Human
  • SwissProt: P21796 Human
  • SwissProt: Q60932 Mouse
  • SwissProt: Q9MZ16 Pig
  • SwissProt: Q9TT15 Rabbit
  • SwissProt: Q9Z2L0 Rat
  • Unigene: 519320 Human
  • Unigene: 709506 Human
  • Unigene: 3555 Mouse
  • Unigene: 54594 Rat

  see all

• Alternative names
  N2441 antibody OMP2 antibody POR1 antibody

  hVDAC1 antibodyOuter mitochondrial membrane protein porin 1 antibodyPlasmalemmal porin antibodyPorin 31HL antibodyPorin 31HM antibodyVDAC antibodyVDAC-1 antibodyVDAC1 antibodyVDAC1_HUMAN antibodyVoltage dependent anion channel 1 antibodyVoltage dependent anion selective channel protein 1 antibodyVoltage-dependent anion-selective channel protein 1 antibodyYNL055C antibodyYNL2441C antibody

  see all

Anti-VDAC/Porin antibody images

• 

  Western blot - VDAC/Porin antibody (ab96140)
  All lanes : Anti-VDAC/Porin antibody (ab96140) at 1 µg/ml
  
  Lane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
  Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 31 kDa
  Observed band size : 31 kDa
  Additional bands at : 44 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 2 minutes
• 

  Immunocytochemistry/ Immunofluorescence - VDAC/Porin antibody (ab96140)

  ICC/IF image of ab96140 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96140 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.