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Immunocytochemistry/ Immunofluorescence - VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control (ab14734)
![Immunocytochemistry/ Immunofluorescence - VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control (ab14734)](/ps/datasheet/images/ab14734_1.jpg)
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Western blot - VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control (ab14734)
![Western blot - VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control (ab14734)](/ps/datasheet/images/ab14734_2.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control(ab14734)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control(ab14734)](\ps\datasheet\images\14\ab14734\VDAC1-Porin-Primary-antibodies-ab14734-1.jpg)
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Product Name
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VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control
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See all VDAC1 / Porin - Loading Control antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [20B12] to VDAC1 / Porin - Mitochondrial Loading Control
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Immunogen
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Recombinant full length protein (Human)
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Reacts with
(species key)
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Hu, Rat
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at a concentration of 0.2 µg/ml.
IF: Use at a concentration of 0.2 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 39 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Outer membranes of all eukaryotic mitochondria
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Research areas
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Isotype/Loading Controls >> Loading Controls >> VDAC1 - Mitochondrial Preps
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Relevance
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The Voltage-Dependent Anion Channel (VDAC or mitochondrial Porin) is an outer membrane mitochondrial protein. The VDAC protein is thought to form the major pores through which adenine nucleotides cross the outer mitochondrial membrane. VDAC has also been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT) and cyclophilin D (CypD) is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. At the mRNA level, the tissue distribution of mitochondrial transport proteins appears to be related to the dependence of each tissue on mitochondrial energy production capacity. As a general rule, these are higher in tissues like skeletal muscle and heart; in liver and pancreas expression levels differ with each protein and in placenta, lung, kidney and brain the expression is lower than in the other tissues.
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Alternative names VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control (ab14734) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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20B12
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Isotype
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IgG2b
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Light chain type
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kappa
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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3.140 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control images, references, products related to ab14734 and other tools.
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VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control images:
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 Immunocytochemistry/ Immunofluorescence - VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control (ab14734)
Immunofluoresence using ab14734 at 0.2 µg/ml on human fibroblasts (red).
Nuclei were labelled with DAPI (blue).
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Western blot - VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control (ab14734)
All lanes : VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control (ab14734) at 1/5000 dilution
Lane 1 : Rat brain cell lysate (homogenate)
Lane 2 : Rat brain cell lysate (homogenate)
Lane 3 : Rat brain cell lysate (mitochondrial)
Lane 4 : Rat brain cell lysate (mitochondrial)
Lysates/proteins at 30 µg per lane.
Secondary
HRP conjugated sheep anti-mouse IgG
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 39 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes This image is courtesy of an anonymous Abreview
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control(ab14734)
Ab14734 staining Human normal left ventricle. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control (ab14734)
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This product has been used in: (two most recent references)
Bivik C et al. Hsp70 protects against UVB induced apoptosis by preventing release of cathepsins and cytochrome c in human melanocytes. Carcinogenesis 28:537-44 (2007). PubMed: 16950797
Hanson BJ et al. An immunocytochemical approach to detection of mitochondrial disorders. J Histochem Cytochem 50:1281-8 (2002). PubMed: 12364561
If you publish research using ab14734 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to VDAC1 / Porin
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VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control - more information
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Customer reviews (feedback) regarding VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control |
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Protocols for VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control |
Price and availability of products related to VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control |
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VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control - related products:
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VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control - other tools:
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Contact Abcam with a Technical Enquiry about VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunocytochemistry/ Immunofluorescence - VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control (ab14734)](/index.html?datasheet=14734#ab14734_1.jpg)
![Western blot - VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control (ab14734)](/index.html?datasheet=14734#ab14734_2.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-VDAC1 / Porin antibody [20B12] - Mitochondrial Loading Control(ab14734)](/index.html?datasheet=14734#VDAC1-Porin-Primary-antibodies-ab14734-1.jpg)
