Publishing research using ab15895? Please let us know so that we can cite the reference in this datasheet
ab15895 has been referenced in 20 publications.
- Mailloux RJ et al. Aluminum toxicity elicits a dysfunctional TCA cycle and succinate accumulation in hepatocytes. J Biochem Mol Toxicol 20:198-208 (2006).
PubMed: 16906525
- Wilhelmus MM et al. Presence of tissue transglutaminase in granular endoplasmic reticulum is characteristic of melanized neurons in Parkinson's disease brain. Brain Pathol 21:130-9 (2011).
IHC-P, IF;
Human.
PubMed: 20731657
- Park D et al. Continued clearance of apoptotic cells critically depends on the phagocyte Ucp2 protein. Nature : (2011).
WB;
Chinese Hamster.
PubMed: 21857682
- Yadav S et al. miR-497 and miR-302b Regulate Ethanol-induced Neuronal Cell Death through BCL2 Protein and Cyclin D2. J Biol Chem 286:37347-57 (2011).
WB;
Human.
PubMed: 21878650
- Bigi A et al. Human sialidase NEU4 long and short are extrinsic proteins bound to outer mitochondrial membrane and the endoplasmic reticulum, respectively. Glycobiology 20:148-57 (2010).
WB;
African Green Monkey.
PubMed: 19797320
- Yang H et al. Analysis of mouse models of cytochrome c oxidase deficiency owing to mutations in Sco2. Hum Mol Genet 19:170-80 (2010).
WB;
Mouse.
PubMed: 19837698
- Morita A et al. Sodium orthovanadate inhibits p53-mediated apoptosis. Cancer Res 70:257-65 (2010).
WB;
Human.
PubMed: 20048077
- Brocker C et al. Aldehyde dehydrogenase 7A1 (ALDH7A1) is a novel enzyme involved in cellular defense against hyperosmotic stress. J Biol Chem 285:18452-63 (2010).
WB;
Mouse.
PubMed: 20207735
- Hoppins S et al. Mitochondrial outer and inner membrane fusion requires a modified carrier protein. J Cell Biol 184:569-81 (2009).
WB;
Mouse.
PubMed: 19237599
- Abu-Hamad S et al. The VDAC1 N-terminus is essential both for apoptosis and the protective effect of anti-apoptotic proteins. J Cell Sci 122:1906-16 (2009).
PubMed: 19461077
- Mailloux RJ et al. Alpha-ketoglutarate abrogates the nuclear localization of HIF-1alpha in aluminum-exposed hepatocytes. Biochimie 91:408-15 (2009).
WB;
Human.
PubMed: 19028544
- Vandermeers F et al. Valproate, in combination with pemetrexed and cisplatin, provides additional efficacy to the treatment of malignant mesothelioma. Clin Cancer Res 15:2818-28 (2009).
WB;
Human.
PubMed: 19351772
- González R et al. N-acetylcysteine, coenzyme Q10 and superoxide dismutase mimetic prevent mitochondrial cell dysfunction and cell death induced by d-galactosamine in primary culture of human hepatocytes. Chem Biol Interact 181:95-106 (2009).
PubMed: 19523936
- Kalifa L et al. Evidence for a role of FEN1 in maintaining mitochondrial DNA integrity. DNA Repair (Amst) 8:1242-9 (2009).
WB;
Mouse.
PubMed: 19699691
- Palmfeldt J et al. Mitochondrial proteomics on human fibroblasts for identification of metabolic imbalance and cellular stress. Proteome Sci 7:20 (2009).
WB;
Human.
PubMed: 19476632
- Lemire J et al. Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1). PLoS ONE 3:e1550 (2008).
WB;
Human.
PubMed: 18253497
- Akbari M et al. Mitochondrial base excision repair of uracil and AP sites takes place by single-nucleotide insertion and long-patch DNA synthesis. DNA Repair (Amst) 7:605-16 (2008).
PubMed: 18295553
- Mori S et al. Expression of Uncoupling Proteins in Human Skin and Skin-Derived Cells. J Invest Dermatol : (2008).
IHC-P;
Human.
PubMed: 18305572
- Westbye MP et al. Human AlkB Homolog 1 Is a Mitochondrial Protein That Demethylates 3-Methylcytosine in DNA and RNA. J Biol Chem 283:25046-56 (2008).
WB;
Human.
PubMed: 18603530
- Vogel RO et al. Human mitochondrial complex I assembly is mediated by NDUFAF1. FEBS J 272:5317-26 (2005).
PubMed: 16218961
Publishing research using ab15895? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - VDAC1 / Porin antibody (ab15895)
All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Hela Nuclear lysate
Lane 2 : Hela cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : Hela Nuclear lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 7 : Hela cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 8 : A431 cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 9 : Jurkat cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 10 : HEK293 cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa
Immunocytochemistry/ Immunofluorescence - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
ICC/IF image of ab15895 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15895, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
Image courtesy of Human Protein Atlas
Paraffin embedded sections of human kidney were incubated with ab15895 (1/600 dilution) at room temperature for 30 mins. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab15895 was also tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org
Western blot - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 6 : Brain (Rat) Tissue Lysate - normal tissue
Lane 7 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
ab15895 staining mouse heart tissue section by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 10% serum for 10 minutes at 25°C. The primary antibody was diluted 1/600 and incubated with the sample for 1 hour at 25°C. A biotinylated goat anti-rabbit antibody diluted 1/400 was used as the secondary.
This image is courtesy of an anonymous Abreview
Western blot - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Adult wild type zebrafish brain mitochondrial lysate at 20 µg
Lane 2 : Adult wild type zebrafish brain cytosolic lysate at 30 µg
Secondary
HRP-conjugated goat F(ab’)2 Fragment at 1/10000 dilution
developed using the ECL technique
Predicted band size : 31 kDa
Observed band size : 28,29 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
Image courtesy of an anonymous Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
ab15895 staining VDAC1 / Porin in human cerebellum tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then incubated with ab15895 at a 1/1000 dilution for 12 hours at 4°C. The secondary used was an undiluted HRP conjugated anti-rabbit polyclonal. Note: The big cells are purkinje cells of the cerebellum, (no nuclear staining).
Image courtesy of Dr Markus Kipp by Abreview.
Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Exposure time : 3 minutes
Western blot image using the Optiblot Reducing Electrophoresis Kit - 10 x 10 cm (4-20%) (ab119220) with the
Prism Ultra Protein Ladder (ab116028) 5µl used. We recommend using our ECL substrate kit (ab65623) .
20ug of Lysate per lane and detection using ab15895 diluted to 1ug/ml.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Lane 4: HEK293 cell lysate
Lane 5: HepG2 cell lysate.
Western blot
All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Chicken liver cell lysate
Lane 2 : CHO K1 cell lysate
Lane 3 : MDCK cell lysate
Lane 4 : Chicken liver cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 5 : CHO K1 cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 6 : MDCK cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa fluor goat polyclonal anti-Rabbit IgG at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa
VDAC1 / Porin antibody - Mitochondrial Loading Control for WB in Human (15895)
VDAC1 / Porin antibody - Mitochondrial Loading Control for WB in Mouse (15895)
VDAC1 / Porin antibody - Mitochondrial Loading Control for IHC-P in Mouse (15895)
VDAC1 / Porin antibody - Mitochondrial Loading Control for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (15895)
Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (15895)
VDAC1 / Porin antibody - Mitochondrial Loading Control for WB in Human (15895)
VDAC1 / Porin antibody - Mitochondrial Loading Control for Western blot in Zebrafish (15895)
VDAC1 / Porin antibody - Mitochondrial Loading Control for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (15895)
VDAC1 / Porin antibody - Mitochondrial Loading Control for Western blot in Human (15895)
Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (15895)
Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (15895)
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