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Western blot
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Western blot - VDAC1 / Porin antibody (ab15895)
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Immunocytochemistry/ Immunofluorescence - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
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Product Name
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VDAC1 / Porin antibody - Mitochondrial Loading Control
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See all VDAC1 / Porin - Loading Control antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to VDAC1 / Porin - Mitochondrial Loading Control
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Immunogen
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Synthetic peptide derived from residues 150 - 250 of Human VDAC1 / Porin.
The immunogen sequence is completely conserved between isoforms 1, 2 and 3 of human VDAC
(Note: the amino acid sequence is proprietary; Peptide available as ab16131.)
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Reacts with
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Hu, Ms, Rat, Chk, ChHm, Dog
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Predicted to react
(species key)
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Cow, Pig, Rb, Zfsh
(Due to sequence homology)
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Specificity
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This antibody detects all 3 isoforms of VDAC.
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Tested applications
(see key)
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ICC/IF, IHC-P, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: 1/600.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). Can be blocked with VDAC1 / Porin peptide (ab16131).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive control in the following human whole cell lysates:
HeLa
A431
Jurkat
HEK293
This antibody gave a positive control in HeLa (Human epithelial carcinoma cell line) Nuclear
This antibody gave a positive control in the following mouse tissue lysates:
Heart
Kidney
Skeletal Muscle
Spinal Cord
This antibody gave a positive control in the following rat lysates:
PC12 Whole Cell
Brain Tissue Lysate - normal tissue
Kidney Whole Cell Lysate - normal tissue
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Cellular localization
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Outer membranes of all eukaryotic mitochondria
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Research areas
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Isotype/Loading Controls >> Loading Controls >> VDAC1 - Mitochondrial Preps
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Relevance
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The Voltage-Dependent Anion Channel (VDAC or mitochondrial Porin) is an outer membrane mitochondrial protein. The VDAC protein is thought to form the major pores through which adenine nucleotides cross the outer mitochondrial membrane. VDAC has also been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT) and cyclophilin D (CypD) is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. At the mRNA level, the tissue distribution of mitochondrial transport proteins appears to be related to the dependence of each tissue on mitochondrial energy production capacity. As a general rule, these are higher in tissues like skeletal muscle and heart; in liver and pancreas expression levels differ with each protein and in placenta, lung, kidney and brain the expression is lower than in the other tissues.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.20 - 0.70 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VDAC1 / Porin antibody - Mitochondrial Loading Control is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VDAC1 / Porin antibody - Mitochondrial Loading Control images, references, products related to ab15895 and other tools.
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VDAC1 / Porin antibody - Mitochondrial Loading Control images:
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 Western blot
All lanes : VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Chicken liver cell lysate
Lane 2 : CHO K1 cell lysate
Lane 3 : MDCK cell lysate
Lane 4 : Chicken liver cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 5 : CHO K1 cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 6 : MDCK cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa fluor goat polyclonal anti-Rabbit IgG at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa
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Western blot - VDAC1 / Porin antibody (ab15895)
All lanes : VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Hela Nuclear lysate
Lane 2 : Hela cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : Hela Nuclear lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 7 : Hela cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 8 : A431 cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 9 : Jurkat cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 10 : HEK293 cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa
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Immunocytochemistry/ Immunofluorescence - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
ICC/IF image of ab15895 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15895, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
Image courtesy of Human Protein Atlas
Paraffin embedded sections of human kidney were incubated with ab15895 (1/600 dilution) at room temperature for 30 mins. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab15895 was also tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org
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Western blot - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
All lanes : VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 6 : Brain (Rat) Tissue Lysate - normal tissue
Lane 7 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
ab15895 staining mouse heart tissue section by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 10% serum for 10 minutes at 25°C. The primary antibody was diluted 1/600 and incubated with the sample for 1 hour at 25°C. A biotinylated goat anti-rabbit antibody diluted 1/400 was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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References for VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
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This product has been used in: (two most recent references)
Bigi A et al. Human sialidase NEU4 long and short are extrinsic proteins bound to outer mitochondrial membrane and the endoplasmic reticulum, respectively. Glycobiology 20:148-57 (2010). WB; African Green Monkey. PubMed: 19797320
Hoppins S et al. Mitochondrial outer and inner membrane fusion requires a modified carrier protein. J Cell Biol 184:569-81 (2009). WB; Mouse. PubMed: 19237599
See all 14 publication references for this product.
If you publish research using ab15895 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to VDAC1 / Porin
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VDAC1 / Porin antibody - Mitochondrial Loading Control - more information
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