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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Overview
Product name
Anti-VDAC1 / Porin antibody
See all VDAC1 / Porin products (4) ...
Description
Rabbit polyclonal to VDAC1 / Porin
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
15 amino acid peptide from near the N terminal of Human VDAC1 / Porin
Positive control
HepG2 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
-
Western blot - VDAC1 / Porin antibody (ab85123)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/200 - 1/500.Predicted molecular weight: 31 kDa.Can be blocked with VDAC1 / Porin peptide (ab95341).
Target
Function
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
Tissue specificity
Heart, liver and skeletal muscle.
Sequence similarities
Belongs to the eukaryotic mitochondrial porin family.
Domain
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
Cellular localization
Mitochondrion outer membrane. Cell membrane.
Target information above from: UniProt accessionP21796
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hVDAC1 antibody
- Outer mitochondrial membrane protein porin 1 antibody
- Plasmalemmal porin antibody
see all
Database links
- Entrez Gene: 7416 Human
- Omim: 604492 Human
- SwissProt: P21796 Human
- Unigene: 519320 Human
- Unigene: 709372 Human
Anti-VDAC1 / Porin antibody images:
Western blot - VDAC1 / Porin antibody (ab85123)
Anti-VDAC1 / Porin antibody (ab85123) at 1/250 dilution + HepG2 cell lysate
Predicted band size : 31 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
References for Anti-VDAC1 / Porin antibody (ab85123)
ab85123 has not yet been referenced specifically in any publications.
Publishing research using ab85123? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"