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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Overview
Product name
VDAC1 / Porin peptide
See all VDAC1 / Porin products (2) ...
Protein description
Synthetic peptide derived from within residues 150 - 250 of Human VDAC1 / Porin.(Note: the amino acid sequence is proprietary)This peptide was used as an immunogen for ab15895 - VDAC1 / Porin antibody - Mitochondrial Loading Control and ab40747 - VDAC1/Porin - HRP antibody (HRP).
Protein length
294 amino acids
Properties
Purity
70 - 90% by HPLC
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Information available upon request.
Concentration
Concentration information loading...
-
Western blot
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking
Application notes
BL: Use at a concentration of 1 µg/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
Tissue specificity
Heart, liver and skeletal muscle.
Sequence similarities
Belongs to the eukaryotic mitochondrial porin family.
Domain
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
Cellular localization
Mitochondrion outer membrane. Cell membrane.
Target information above from: UniProt accessionP21796
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
VDAC1 / Porin peptide images:
Western blot
All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Chicken liver cell lysate
Lane 2 : CHO K1 cell lysate
Lane 3 : MDCK cell lysate
Lane 4 : Chicken liver cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 5 : CHO K1 cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 6 : MDCK cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa fluor goat polyclonal anti-Rabbit IgG at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa
References for VDAC1 / Porin peptide (ab16131)
ab16131 has not yet been referenced specifically in any publications.
Publishing research using ab16131? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"