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Overview

  • Product nameVDAC1 / Porin peptideSee all VDAC1 / Porin proteins and peptides ...
  • Protein descriptionSynthetic peptide derived from within residues 150 - 250 of Human VDAC1 / Porin.(Note: the amino acid sequence is proprietary)This peptide was used as an immunogen for ab15895 - VDAC1 / Porin antibody - Mitochondrial Loading Control and ab40747 - VDAC1/Porin - HRP antibody (HRP).
  • Protein length294 amino acids

    • Properties

  • Purity70 - 90% by HPLC
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferInformation available upon request.
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab16131 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Blocking
  • Application notesBL: Use at a concentration of 1 µg/ml.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        hVDAC1MGC111064Mitochondrial Porin
        Outer mitochondrial membrane protein porin 1Plasmalemmal porinPorinPorin 31HLPorin 31HMPORIN-31-HLVDACVDAC 1VDAC-1Vdac1VDAC1_HUMANVoltage Dependent Anion Channel 1Voltage dependent anion selective channel protein 1Voltage-dependent anion-selective channel protein 1
      see all
  • FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
  • Tissue specificityHeart, liver and skeletal muscle.
  • Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
  • DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
  • Cellular localizationMitochondrion outer membrane. Cell membrane.

    • Target information above from: UniProt accession P21796 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    VDAC1 / Porin peptide images

    • Western blot
      Western blot
      All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml

      Lane 1 : Chicken liver cell lysate
      Lane 2 : CHO K1 cell lysate
      Lane 3 : MDCK cell lysate
      Lane 4 : Chicken liver cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
      Lane 5 : CHO K1 cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml
      Lane 6 : MDCK cell lysate with VDAC1 / Porin peptide (ab16131) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      Alexa fluor goat polyclonal anti-Rabbit IgG at 1/10000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 31 kDa
      Observed band size : 31 kDa

    References for VDAC1 / Porin peptide (ab16131)

    ab16131 has not yet been referenced specifically in any publications.

    Publishing research using ab16131 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    Thank you for information about the new products. I have a question about the Porin peptide (ab16131). What is this for? Inhibitory peptide? I could Not find the functional information about it through your website.. Could you tell me what it is ? ...

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    Thank you for your enquiry and your interest in our products. We would like to confirm that ab16131 is a blocking peptide which can be used with ab3434:VDAC1 / Porin primary antibody for Western blot application.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"