HRP Anti-VDAC1/Porin antibody [N152B/23] (ab186337)
Key features and details
- HRP Mouse monoclonal [N152B/23] to VDAC1/Porin
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Conjugation: HRP
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
HRP Anti-VDAC1/Porin antibody [N152B/23]
See all VDAC1/Porin primary antibodies -
Description
HRP Mouse monoclonal [N152B/23] to VDAC1/Porin -
Host species
Mouse -
Conjugation
HRP -
Specificity
Does not cross-react with VDAC2 or VDAC3 (based on KO validation results).
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human VDAC1/Porin aa 1 to the C-terminus.
Database link: P21796 -
Positive control
- WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
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General notes
The clone number has been updated from S152B-23 to N152B/23, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N152B/23 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. -
Tissue specificity
Heart, liver and skeletal muscle. -
Sequence similarities
Belongs to the eukaryotic mitochondrial porin family. -
Domain
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity. -
Cellular localization
Mitochondrion outer membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7416 Human
- Entrez Gene: 83529 Rat
- Omim: 604492 Human
- SwissProt: P21796 Human
- SwissProt: Q9Z2L0 Rat
- Unigene: 519320 Human
- Unigene: 709506 Human
- Unigene: 54594 Rat
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Alternative names
- N2441 antibody
- OMP2 antibody
- POR1 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab186337 has not yet been referenced specifically in any publications.