Anti-VDAC1/Porin antibody [N152B/23] (ab186321)
Key features and details
- Mouse monoclonal [N152B/23] to VDAC1/Porin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-VDAC1/Porin antibody [N152B/23]
See all VDAC1/Porin primary antibodies -
Description
Mouse monoclonal [N152B/23] to VDAC1/Porin -
Host species
Mouse -
Specificity
ab186321 shows weak cross reactivity with VDAC3.
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Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human VDAC1/Porin aa 1 to the C-terminus.
Database link: P21796 -
Positive control
- WB: HEK-293, HAP1, and MEF cell lysates. Rat brain tissue lysate. N-GST tagged Recombinant Human VDAC1 protein. ICC/IF: SH-SY5Y cells. IHC-P: Human tonsil, Human breast carcinoma, Rat Kidney, and Mouse kidney tissues.
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General notes
The clone number has been updated from S152B-23 to N152B/23, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N152B/23 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 31 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/100.
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Notes |
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WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 31 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Target
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Function
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. -
Tissue specificity
Heart, liver and skeletal muscle. -
Sequence similarities
Belongs to the eukaryotic mitochondrial porin family. -
Domain
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity. -
Cellular localization
Mitochondrion outer membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7416 Human
- Entrez Gene: 22333 Mouse
- Entrez Gene: 83529 Rat
- Omim: 604492 Human
- SwissProt: P21796 Human
- SwissProt: Q60932 Mouse
- SwissProt: Q9Z2L0 Rat
- Unigene: 519320 Human
see all -
Alternative names
- N2441 antibody
- OMP2 antibody
- POR1 antibody
see all
Images
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Immunocytochemical analysis of 4% Paraformaldehyde-fixed human SH-SY5Y cells labelling VDAC1 with ab186321 at 1/100 dilution, followed by a secondary antibody (Alexa Fluor® 488) at 1/1000 dilution for 1 hour at room temperature.
Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VDAC1 Antibody (D) Composite.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling VDAC1 with ab186321 at 1/1000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection) at the supplied concentration. Dot staining on human tonsil is observed. Counter stained with hematoxylin. The section was incubated with ab186321 for 30 mins at room temperature, followed by an anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: PBS used instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
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All lanes : Anti-VDAC1/Porin antibody [N152B/23] (ab186321) at 1/1000 dilution
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line) whole cell lysate
Lane 3 : MEF (Mus musculus Embryo Fibroblast) whole cell lysate
Lane 4 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking peptide and diluting buffer: 5% NFDM /TBST
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All lanes : Anti-VDAC1/Porin antibody [N152B/23] (ab186321) at 1/1000 dilution
Lane 1 : N-GST tagged Recombinant Human VDAC1 (aa1 to 283) protein
Lane 2 : N-GST tagged Recombinant Human VDAC2 (aa 1 to 294) protein
Lane 3 : N-GST tagged Recombinant Human VDAC3 (aa 1 to 283) protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Exposure time: 80 secondsN-GST tagged Recombinant Human VDAC1 protein is available as ab132481
N-GST tagged Recombinant Human VDAC2 protein is available as ab152793
Blocking peptide and diluting buffer: 5% NFDM /TBST
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling VDAC1 with ab186321 at 1/1000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection) at the supplied concentration. Dot staining on human breast carcinoma is observed. Counter stained with hematoxylin. The section was incubated with ab186321 for 30 mins at room temperature, followed by an anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: PBS used instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling VDAC1 with ab186321 at 1/1000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection) at the supplied concentration. Dot staining on mouse kidney is observed. Counter stained with hematoxylin. The section was incubated with ab186321 for 30 mins at room temperature, followed by an anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: PBS used instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue labelling VDAC1 with ab186321 at 1/1000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection) at the supplied concentration. Dot staining on rat kidney is observed. Counter stained with hematoxylin. The section was incubated with ab186321 for 30 mins at room temperature, followed by an anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: PBS used instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab186321 has been referenced in 6 publications.
- Lu X et al. Ultrastructural and proteomic profiling of mitochondria-associated endoplasmic reticulum membranes reveal aging signatures in striated muscle. Cell Death Dis 13:296 (2022). PubMed: 35368021
- Buelli S et al. Shiga Toxin 2 Triggers C3a-Dependent Glomerular and Tubular Injury through Mitochondrial Dysfunction in Hemolytic Uremic Syndrome. Cells 11:N/A (2022). PubMed: 35681450
- Locatelli M et al. Sirtuin 3 Deficiency Aggravates Kidney Disease in Response to High-Fat Diet through Lipotoxicity-Induced Mitochondrial Damage. Int J Mol Sci 23:N/A (2022). PubMed: 35955472
- Bloch NB et al. The conformational stability of pro-apoptotic BAX is dictated by discrete residues of the protein core. Nat Commun 12:4932 (2021). PubMed: 34389733
- Morigi M et al. C3a receptor blockade protects podocytes from injury in diabetic nephropathy. JCI Insight 5:N/A (2020). PubMed: 32161193
- Milenkovic VM et al. CRISPR-Cas9 Mediated TSPO Gene Knockout alters Respiration and Cellular Metabolism in Human Primary Microglia Cells. Int J Mol Sci 20:N/A (2019). PubMed: 31323920