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Products:Signal Transduction >> Metabolism >> Mitochondrial
Overview
Product name
Anti-VDAC2 antibody
See all VDAC2 products (5) ...
Description
Goat polyclonal to VDAC2
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Rabbit, Cow, Pig
Immunogen
Synthetic peptide: C-GHKVGLALELEA, corresponding to C terminal amino acids 283-294 of Human VDAC2
C-GHKVGLAL ELEA
Positive control
Human Brain and Testes lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.3 - 1 µg/ml.Predicted molecular weight: 32 kDa.
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
Tissue specificity
Expressed in all tissues examined.
Sequence similarities
Belongs to the eukaryotic mitochondrial porin family.
Domain
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.
Cellular localization
Mitochondrion outer membrane.
Target information above from: UniProt accessionP45880
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B-36 vdac antibody
- FLJ23841 antibody
- hVDAC2 antibody
see all
Database links
- Entrez Gene: 282120 Cow
- Entrez Gene: 7417 Human
- Entrez Gene: 22334 Mouse
- Entrez Gene: 397659 Pig
- Entrez Gene: 83531 Rat
- NCBI: NP_003366 Human
- Omim: 193245 Human
- SwissProt: P68002 Cow
see all
Anti-VDAC2 antibody images:
Western blot - VDAC2 antibody (ab37985)
Anti-VDAC2 antibody (ab37985) at 1 µg/ml + Human Testis lysate (35µg protein in RIPA buffer).
Predicted band size : 32 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by
chemiluminescence.
Immunocytochemistry/ Immunofluorescence-VDAC2 antibody(ab37985)
ICC/IF image of ab37985 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37985, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-VDAC2 antibody (ab37985)
This product has been referenced in:
- Zheng JFet al. Hippocampal protein levels related to spatial memory are different in the Barnes maze and in the multiple T-maze. J Proteome Res 8:4479-86 (2009).Read more (PubMed: 19650667) »
- Sarioglu Het al. Analysis of 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced proteome changes in 5L rat hepatoma cells reveals novel targets of dioxin action including the mitochondrial apoptosis regulator VDAC2. Mol Cell Proteomics 7:394-410 (2008).Read more (PubMed: 17998243) »
See all 2 publications for this product
Publishing research using ab37985? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"