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Western blot - VDAC2 antibody (ab37985)
(enlarge)
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Product Name
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VDAC2 antibody
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See all VDAC2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to VDAC2
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Immunogen
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Synthetic peptide: C-GHKVGLALELEA, corresponding to C terminal amino acids 298/309 of Human VDAC2
BLAST 'C-GHKVGLALELEA' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Cow, Pig, Rb
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1/1000.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 38 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Brain and Testes lysates.
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Cellular localization
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Outer mitochondrial membrane.
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Research areas
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Signal Transduction >> Metabolism >> Mitochondrial
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Relevance
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VDAC2 forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
VDAC2 interacts with hexokinases. It is present in the outer mitochondrial membrane. There are 4 isoforms as result of alternative splicing. It consists mainly of membrane spanning sided beta sheets, and belongs to the eukaryotic mitochondrial porin family.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VDAC2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VDAC2 antibody images, references, products related to ab37985 and other tools.
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VDAC2 antibody images:
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 Western blot - VDAC2 antibody (ab37985)
VDAC2 antibody (ab37985) at 1 µg/ml + Human Testis lysate (35µg protein in RIPA buffer).
Predicted band size : 38 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by
chemiluminescence.
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References for VDAC2 antibody (ab37985)
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This product has been used in:
Sarioglu H et al. Analysis of 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced proteome changes in 5L rat hepatoma cells reveals novel targets of dioxin action including the mitochondrial apoptosis regulator VDAC2. Mol Cell Proteomics 7:394-410 (2008). PubMed: 17998243
If you publish research using ab37985 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to VDAC2
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VDAC2 antibody - more information
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Customer reviews (feedback) regarding VDAC2 antibody |
Customer FAQs regarding VDAC2 antibody |
Protocols for VDAC2 antibody |
Price and availability of products related to VDAC2 antibody |
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VDAC2 antibody - related products:
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VDAC2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about VDAC2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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