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ab47104 has been referenced in 6 publications.
Publishing research using ab47104? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-VDAC2 antibody (ab47104) at 2 µg/ml
Lane 1 : Human brain cell lysate with no immunizing peptide
Lane 2 : Human brain cell lysate with immunizing peptide
Predicted band size : 32 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
The approximately 55 kD band is not blocked by the immunizing peptide and is believed to result from non-specific cross-reaction.
IHC image of ab47104 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47104, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-VDAC2 antibody (ab47104) at 2 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Human heart mitochondrial extract at 20 µg
Secondary
Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 32 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
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