You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-VDAC2 antibody (ab47104)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab47104 for help.

Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.

1 question for ab47104

first page       

Page 1 of 1

      last page  

Question 1

Thursday 06-September-2007

On the datasheet, it is stated that VDAC3 can also be detected but VDAC2 molecular weight is 38kDa and VDAC3 is 31kDa, and it is strange to only have one band on the WB image. Can this product really cross react with VDAC3?

ANSWER:

  

Thank you for your enquiry.

The recognition of VDAC3 is because on the homology betweeen VDAC2 and VDAC3 (peptide immunogen sequence listed on datashee). Thus, a polyclonal antibody made against the particular VDAC sequence would be expected to recognize VDAC3 based on the homology with the VDAC3 sequence. There would be more than one band in the western blot if the testing material expressed both proteins. Otherwise to confirm, one could use recombinant VDAC2 and VDAC3, but unfortunately we have not done this. The detection of two bands, can also depend on gel conditions designed to separate two closely migrating bands. Additionally, proteins can have various post-translational modifications which can alter their molecular weights. Therefore, the observed band could represent VDAC2 or VDAC3 and that is why we refer to it as VDAC2/3. Further experimentation would need to be done to determine the exact identity of the band.

first page       

Page 1 of 1

      last page  

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"