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Products:Signal Transduction >> Metabolism >> Mitochondrial
Overview
Product name
Anti-VDAC2 antibody
See all VDAC2 products (5) ...
Description
Rabbit polyclonal to VDAC2
Specificity
This antibody also reconginizes VDAC3 with which its sequence is 76% homologous.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Cow, Cat, Dog, Human, Chimpanzee, Zebrafish
Predicted to work with
Mouse, Rat, Rabbit, Chicken, Turkey, Pig
Immunogen
Synthetic peptide: KKSGKIKSSYKRE, corresponding to internal sequence amino acids 120-132 of Human VDAC2
KKSGKIKSSY KRE
Positive control
Human brain cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 3 µg/ml.Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
Tissue specificity
Expressed in all tissues examined.
Sequence similarities
Belongs to the eukaryotic mitochondrial porin family.
Domain
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.
Cellular localization
Mitochondrion outer membrane.
Target information above from: UniProt accessionP45880
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B-36 vdac antibody
- FLJ23841 antibody
- hVDAC2 antibody
see all
Database links
- Entrez Gene: 395498 Chicken
- Entrez Gene: 282120 Cow
- Entrez Gene: 479255 Dog
- Entrez Gene: 7417 Human
- Entrez Gene: 22334 Mouse
- Entrez Gene: 397659 Pig
- Entrez Gene: 83531 Rat
- Entrez Gene: 322126 Zebrafish
see all
Anti-VDAC2 antibody images:
Western blot - VDAC2 antibody (ab47104)
All lanes : Anti-VDAC2 antibody (ab47104) at 2 µg/ml
Lane 1 : Human brain cell lysate with no immunizing peptide
Lane 2 : Human brain cell lysate with immunizing peptide
Predicted band size : 32 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
The approximately 55 kD band is not blocked by the immunizing peptide and is believed to result from non-specific cross-reaction.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VDAC2 antibody (ab47104)
IHC image of ab47104 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47104, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-VDAC2 antibody (ab47104)
All lanes : Anti-VDAC2 antibody (ab47104) at 2 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Human heart mitochondrial extract at 20 µg
Secondary
Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 32 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
References for Anti-VDAC2 antibody (ab47104)
This product has been referenced in:
- Mutsaers CAet al. Reversible molecular pathology of skeletal muscle in spinal muscular atrophy. Hum Mol Genet 20:4334-44 (2011).Read more (PubMed: 21840928) »
- Chiao YAet al. In vivo matrix metalloproteinase-7 substrates identified in the left ventricle post-myocardial infarction using proteomics. J Proteome Res 9:2649-57 (2010). WB; Mouse.Read more (PubMed: 20232908) »
See all 5 publications for this product
Publishing research using ab47104? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"