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Anti-VDAC2 antibody (ab77160) at 1 µg/ml + GST-tagged recombinant full length Human VDAC2 at 0.1 µg
Secondary
Goat Anti-Mouse IgG (H&L) HRP.conjugated at 1/5000 dilution
Predicted band size : 32 kDa
Observed band size : 54 kDa (why is the actual band size different from the predicted?)
MW of GST tag: 26 kDa
All lanes : Anti-VDAC2 antibody (ab77160) at 5 µg/ml
Lane 1 : VDAC2-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size : 32 kDa
Observed band size : 32 kDa
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