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Western blot - VDAC2 antibody (ab77160)
(enlarge)
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Western blot - VDAC2 antibody (ab77160)
(enlarge)
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Product Name
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VDAC2 antibody
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See all VDAC2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to VDAC2
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Immunogen
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GST-tagged recombinant full length Human protein (AAH00165)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 38 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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VDAC2-transfected 293T cell lysate.
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Cellular localization
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Outer mitochondrial membrane.
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Research areas
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Signal Transduction >> Metabolism >> Mitochondrial
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Relevance
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VDAC2 forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
VDAC2 interacts with hexokinases. It is present in the outer mitochondrial membrane. There are 4 isoforms as result of alternative splicing. It consists mainly of membrane spanning sided beta sheets, and belongs to the eukaryotic mitochondrial porin family.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VDAC2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VDAC2 antibody images, references, products related to ab77160 and other tools.
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VDAC2 antibody images:
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 Western blot - VDAC2 antibody (ab77160)
VDAC2 antibody (ab77160) at 1 µg/ml + GST-tagged recombinant full length Human VDAC2 at 0.1 µg
Secondary
Goat Anti-Mouse IgG (H&L) HRP.conjugated at 1/5000 dilution
Predicted band size : 38 kDa
Observed band size : 54 kDa (why is the actual band size different from the predicted?)
MW of GST tag: 26 kDa
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Western blot - VDAC2 antibody (ab77160)
All lanes : VDAC2 antibody (ab77160) at 5 µg/ml
Lane 1 : VDAC2-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size : 38 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to VDAC2
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VDAC2 antibody - more information
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Customer reviews (feedback) regarding VDAC2 antibody |
Customer FAQs regarding VDAC2 antibody |
Protocols for VDAC2 antibody |
Price and availability of products related to VDAC2 antibody |
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VDAC2 antibody - related products:
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VDAC2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about VDAC2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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