Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Metabolism >> Mitochondrial
Overview
Product name
Anti-VDAC2 antibody
See all VDAC2 products (5) ...
Description
Mouse monoclonal to VDAC2
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
GST-tagged recombinant full length Human protein (AAH00165)
Positive control
VDAC2-transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 32 kDa.
ELISA: Use at an assay dependent dilution.
Target
Function
Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
Tissue specificity
Expressed in all tissues examined.
Sequence similarities
Belongs to the eukaryotic mitochondrial porin family.
Domain
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.
Cellular localization
Mitochondrion outer membrane.
Target information above from: UniProt accessionP45880
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B-36 vdac antibody
- FLJ23841 antibody
- hVDAC2 antibody
see all
Database links
- Entrez Gene: 7417 Human
- NCBI: NP_003366 Human
- Omim: 193245 Human
- SwissProt: P45880 Human
- SwissProt: Q5VWK1 Human
- SwissProt: Q6IB40 Human
- SwissProt: Q9BWK8 Human
- Unigene: 355927 Human
Anti-VDAC2 antibody images:
Western blot - Anti-VDAC2 antibody (ab77160)
Anti-VDAC2 antibody (ab77160) at 1 µg/ml + GST-tagged recombinant full length Human VDAC2 at 0.1 µg
Secondary
Goat Anti-Mouse IgG (H&L) HRP.conjugated at 1/5000 dilution
Predicted band size : 32 kDa
Observed band size : 54 kDa (why is the actual band size different from the predicted?)
MW of GST tag: 26 kDa
Western blot - VDAC2 antibody (ab77160)
All lanes : Anti-VDAC2 antibody (ab77160) at 5 µg/ml
Lane 1 : VDAC2-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size : 32 kDa
Observed band size : 32 kDa
References for Anti-VDAC2 antibody (ab77160)
This product has been referenced in:
- Zhou Ret al. A role for mitochondria in NLRP3 inflammasome activation. Nature 469:221-5 (2011). WB; Human.Read more (PubMed: 21124315) »
See 1 publication for this product
Publishing research using ab77160? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"