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Western blot - VDAC3 antibody (ab80452)
(enlarge)
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Product Name
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VDAC3 antibody
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See all VDAC3 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to VDAC3
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Immunogen
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Synthetic peptide: TQKWNTDNTL GTEISWENKL AEGLKLTLDT IFVPNTGKKS GKLKASYKRD , corresponding to N terminal amino acids 73-122 of Human VDAC3 (NP_005653)
BLAST 'TQKWNTDNTL GTEISWENKL AEGLKLTLDT IFVPNTGKKS GKLKASYKRD ' with  or 
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat, Cow, Pig, Rb, Orngt
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay: 1/62500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Fetal heart lysate.
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Cellular localization
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Mitochondrial outer membrane.
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Research areas
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Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Cell Biology >> Apoptosis >> Mitochondrial
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Relevance
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The Voltage-Dependant Anion Channel (VDAC3) of the outer mitochondrial membrane, a small abundant protein found in all eukaryotic kingdoms, forms a voltage-gated pore when incorporated into planar lipid bilayers. VDAC is also the site of binding of the metabolic enzymes hexokinase and glycerol kinase to the mitochondrion in what may be a significant metabolic regulatory interaction. VDAC3 is involved specifically in translocation of adenine nucleotides through the outer membrane.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Purification notes
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ab80452 is purified by a peptide affinity chromatography method.
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Form
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Lyophilised
Add 50 µl of distilled water.
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VDAC3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VDAC3 antibody images, references, products related to ab80452 and other tools.
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VDAC3 antibody images:
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 Western blot - VDAC3 antibody (ab80452)
VDAC3 antibody (ab80452) at 1 µg/ml + Fetal heart lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 31 kDa
Observed band size : 31 kDa
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Search PubMed (MEDLINE) for references to VDAC3
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VDAC3 antibody - more information
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Customer reviews (feedback) regarding VDAC3 antibody |
Customer FAQs regarding VDAC3 antibody |
Protocols for VDAC3 antibody |
Price and availability of products related to VDAC3 antibody |
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VDAC3 antibody - related products:
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VDAC3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about VDAC3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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