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Overview

  • Product nameVDAC3 protein (Tagged-His Tag)See all VDAC3 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 102-179 of Human VDAC3 with N terminal His tag; MWt 9kDa (UniProt Q9Y277).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 101 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesIFVPNTGKKSGKLKASYKRDCFSVGSNVDIDFSGPTIYGW AVLAFEGWLAGYQM SFDTAKSKLSQNNFALGYKAADFQ
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91743 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        hVDAC3mVDAC3Outer mitochondrial membrane protein porin 3voltage dependent anion channel 3
  • RelevanceThe Voltage-Dependant Anion Channel (VDAC3) of the outer mitochondrial membrane, a small abundant protein found in all eukaryotic kingdoms, forms a voltage-gated pore when incorporated into planar lipid bilayers. VDAC is also the site of binding of the metabolic enzymes hexokinase and glycerol kinase to the mitochondrion in what may be a significant metabolic regulatory interaction. VDAC3 is involved specifically in translocation of adenine nucleotides through the outer membrane.
  • Cellular localizationMitochondrial outer membrane.

    • VDAC3 protein (Tagged-His Tag) images

    • SDS-PAGE - VDAC3 protein (Tagged-His Tag) (ab91743)
      SDS-PAGE - VDAC3 protein (Tagged-His Tag) (ab91743)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for VDAC3 protein (Tagged-His Tag) (ab91743)

    ab91743 has not yet been referenced specifically in any publications.

    Publishing research using ab91743 ? Please let us know so that we can cite the reference in this datasheet

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