![Immunocytochemistry/ Immunofluorescence - VE Cadherin antibody [BV9] (ab7047) image](#ab7047_1.jpg)
![Flow Cytometry - VE Cadherin antibody [BV9] (ab7047) image](#VE-Cadherin-Primary-antibodies-ab7047-4.jpg)
![Immunocytochemistry/ Immunofluorescence - VE Cadherin antibody [BV9] (ab7047) image](#VE-Cadherin-Primary-antibodies-ab7047-5.jpg)
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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins
Overview
Product name
Anti-VE Cadherin antibody [BV9]
See all VE Cadherin products (10) ...
Description
Mouse monoclonal [BV9] to VE Cadherin
Specificity
This monoclonal antibody (subclass IgG2a) binds to human VE-cadherin. Human vascular endothelium cadherin is an endothelium-specific cadherin localized at the intercellular junctions.
Tested applications
ICC, WB, IP, IHC-Fr, Flow Cyt, ICC/IF, IHC-P, Functional Studiesmore details
Cross reactivity
Reacts with
Mouse, Human
Epitope
The antibody recognizes the extracellular domain of the protein.
Positive control
HUVECs grown on coverslips
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, 0.2% BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
BV9
Isotype
IgG2a
Light chain type
kappa
Research areas
Cardiovascular >> Cardiovascular Markers >> Cell Markers >> Endothelial Cells
Cardiovascular >> Vasculature >> Endothelium
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Cadherins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Vascular
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC
ICC: 1/50ICC: 1/50
WB: 1/50Detects a band of approximately 130-140 kDa (predicted molecular weight: 88 kDa).
IP
IP: 1/50IP: 1/50
IHC-Fr: 1/50
Flow Cyt: 1/50
ICC/IF: 1/50
IHC-P: Use at an assay dependent dilution. (PubMed: 19571314)
FuncS: Use at an assay dependent dilution. (Antibody BV9 functions as an antagonist. The antibody was functionally tested by adding 10-50µg/ml antibody BV9 to cell culture. The antibody blocks VE-cadherin causing a redistribution of VE-cadherin away from intracellular junctions.)
Target
Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
Tissue specificity
Endothelial tissues and brain.
Sequence similarities
Contains 5 cadherin domains.
Post-translational
modifications
Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.
Cellular localization
Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
Target information above from: UniProt accessionP33151
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 7B 4 antibody
- 7B4 antibody
- 7B4 antigen antibody
see all
Database links
- Entrez Gene: 1003 Human
- Entrez Gene: 12562 Mouse
- Omim: 601120 Human
- SwissProt: P33151 Human
- SwissProt: Q4VAI4 Human
- SwissProt: Q4VAI5 Human
- SwissProt: Q4VAI6 Human
- SwissProt: P55284 Mouse
see all
Anti-VE Cadherin antibody [BV9] images:
Immunocytochemistry/ Immunofluorescence - VE Cadherin antibody [BV9] (ab7047)
![Immunocytochemistry/ Immunofluorescence - VE Cadherin antibody [BV9] (ab7047)](/ps/datasheet/Images/7/ab7047/ab7047_1.jpg)
A confocal microscopy image of Endothelial cells from the breast expressing VE-cadherin. This image was kindly submitted as part of the review by Valgardur Sigurdsson.
Flow Cytometry - VE Cadherin antibody [BV9] (ab7047)
![Flow Cytometry - VE Cadherin antibody [BV9] (ab7047)](/ps/datasheet/images/7/ab7047/VE-Cadherin-Primary-antibodies-ab7047-4.jpg)
Overlay histogram of HUVEC cells stained with antibody ab7047 in PBS/0.1% saponin for 1 hours at 4°C.(Black line - no stain, Red line - isotype control, Blue- ab7047).
Immunocytochemistry/ Immunofluorescence - VE Cadherin antibody [BV9] (ab7047)
![Immunocytochemistry/ Immunofluorescence - VE Cadherin antibody [BV9] (ab7047)](/ps/datasheet/images/7/ab7047/VE-Cadherin-Primary-antibodies-ab7047-5.jpg)
ab7047 staining VE Cadherin in endothelial cells by Immunocytochemistry/ Immunofluorescence. Endothelial cells were fixed with 4% PAF (15 minutes, room temperature), and then permeabilized with 0.5% Triton X-100 (3 minutes, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa Fluor® 647 and counterstained using DAPI.
References for Anti-VE Cadherin antibody [BV9] (ab7047)
This product has been referenced in:
- Wu Tet al. Functional expression of P2X4 receptor in capillary endothelial cells of the cochlear spiral ligament and its role in regulating the capillary diameter. Am J Physiol Heart Circ Physiol 301:H69-78 (2011). IHC-FrFl; Guinea pig.Read more (PubMed: 21460192) »
- Almagro Set al. The motor protein myosin-X transports VE-cadherin along filopodia to allow the formation of early endothelial cell-cell contacts. Mol Cell Biol 30:1703-17 (2010). WB; Human.Read more (PubMed: 20123970) »
See all 10 publications for this product
Publishing research using ab7047? Please let us know so that we can cite the reference in this datasheet
![Anti-VE Cadherin antibody [BV9] for Western blot in Human (7047)](/ps/datasheet/images/7/ab7047/VE-Cadherin-Primary-antibodies-ab7047-12.JPG)
![VE Cadherin antibody [BV9] for ICC/IF in Human (7047)](/ps/Reviews/Images/ab10523_55237.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"