Anti-VE Cadherin (phospho Y658) antibody (ab27775)
- Product nameAnti-VE Cadherin (phospho Y658) antibodySee all VE Cadherin primary antibodies ...
- DescriptionRabbit polyclonal to VE Cadherin (phospho Y658)
- Specificityab27775 recognises VE-cadherin [pY658].
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Chimpanzee
Synthetic phosphopeptide - derived from the region of human VE cadherin that contains tyrosine 658.
- Positive control
- HUVEC cells treated with VEGF for 10 minutes.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, Dulbecco's PBS[magnesium and calcium free], 1.0mg/ml BSA (IgG, protease free). pH 7.3
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesPurified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VE-cadherin. The final product is generated by affinity chromatography using an VE-cadherin-derived peptide that is phosphorylated at tyrosine 658.
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab27775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/1000. Predicted molecular weight: 88 kDa.|
- FunctionCadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
- Tissue specificityEndothelial tissues and brain.
- Sequence similaritiesContains 5 cadherin domains.
modificationsPhosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.
- Cellular localizationCell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
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Anti-VE Cadherin (phospho Y658) antibody images
All lanes : Anti-VE Cadherin (phospho Y658) antibody (ab27775) at 1/1000 dilution
Lane 1 : HUVEC cell extract NOT treated with VEGF with NO peptide added
Lane 2 : HUVEC cell extract treated with 100ng/ml VEGF and with NO peptide added
Lane 3 : HUVEC cell extract treated with 100ng/ml VEGF and with a non phosphopeptide corresponding to the immunogen.
Lane 4 : HUVEC cell extract treated with 100ng/ml VEGF and with a generic phosphotyrosine containing peptide.
Lane 5 : HUVEC cell extract treated with 100ng/ml VEGF and with the immunogen
Goat F(ab')2 anti rabbit IgG.
Performed under reducing conditions.
Predicted band size : 88 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
References for Anti-VE Cadherin (phospho Y658) antibody (ab27775)
This product has been referenced in:
- Rodríguez MI et al. PARP-1 regulates metastatic melanoma through modulation of vimentin-induced malignant transformation. PLoS Genet 9:e1003531 (2013). WB . Read more (PubMed: 23785295) »