Anti-VE Cadherin (phospho Y658) antibody (ab27775)

Overview

  • Product nameAnti-VE Cadherin (phospho Y658) antibodySee all VE Cadherin primary antibodies ...
  • Description
    Rabbit polyclonal to VE Cadherin (phospho Y658)
  • Specificityab27775 recognises VE-cadherin [pY658].
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Chimpanzee
  • Immunogen

    Synthetic phosphopeptide - derived from the region of human VE cadherin that contains tyrosine 658.

  • Positive control
    • HUVEC cells treated with VEGF for 10 minutes.

Properties

Applications

Our Abpromise guarantee covers the use of ab27775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24812668
WB 1/1000. Predicted molecular weight: 88 kDa.

Target

  • FunctionCadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
  • Tissue specificityEndothelial tissues and brain.
  • Sequence similaritiesContains 5 cadherin domains.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.
  • Cellular localizationCell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • Database links
  • Alternative names
    • 7B 4 antibody
    • 7B4 antibody
    • 7B4 antigen antibody
    • CADH5_HUMAN antibody
    • Cadherin 5 antibody
    • Cadherin 5 antibody
    • Cadherin 5 type 2 antibody
    • Cadherin 5, type 2 (vascular endothelium) antibody
    • Cadherin 5, type 2, VE cadherin (vascular epithelium) antibody
    • cadherin, vascular endothelial antibody
    • cadherin, vascular endothelial, 1 antibody
    • Cadherin-5 antibody
    • Cadherin5 antibody
    • CD 144 antibody
    • CD144 antibody
    • CD144 antigen antibody
    • CDH 5 antibody
    • CDH5 antibody
    • CDH5 protein antibody
    • Endothelial specific cadherin antibody
    • FLJ17376 antibody
    • OTTHUMP00000174777 antibody
    • Vascular endothelial cadherin antibody
    • Vascular epithelium cadherin antibody
    • VE Cad antibody
    • VE-cadherin antibody
    • VEC antibody
    see all

Anti-VE Cadherin (phospho Y658) antibody images

  • All lanes : Anti-VE Cadherin (phospho Y658) antibody (ab27775) at 1/1000 dilution

    Lane 1 : HUVEC cell extract NOT treated with VEGF with NO peptide added
    Lane 2 : HUVEC cell extract treated with 100ng/ml VEGF and with NO peptide added
    Lane 3 : HUVEC cell extract treated with 100ng/ml VEGF and with a non phosphopeptide corresponding to the immunogen.
    Lane 4 : HUVEC cell extract treated with 100ng/ml VEGF and with a generic phosphotyrosine containing peptide.
    Lane 5 : HUVEC cell extract treated with 100ng/ml VEGF and with the immunogen

    Secondary
    Goat F(ab')2 anti rabbit IgG.

    Performed under reducing conditions.

    Predicted band size : 88 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

References for Anti-VE Cadherin (phospho Y658) antibody (ab27775)

This product has been referenced in:
  • Cruz-Orengo L  et al. Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility. J Clin Invest 124:2571-84 (2014). ICC/IF ; Mouse . Read more (PubMed: 24812668) »
  • Rodríguez MI  et al. PARP-1 regulates metastatic melanoma through modulation of vimentin-induced malignant transformation. PLoS Genet 9:e1003531 (2013). WB . Read more (PubMed: 23785295) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (7%)
Sample Mouse Cell lysate - whole cell (lung endothelium)
Specification lung endothelium
Treatment 30 nM VEGF for 10 min
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Stephen Robinson

Verified customer

Submitted Aug 04 2014

Thank you for your inquiry.

I am happy to confirm that both antibodies are tested and guaranteed for human sample in WB.

I would like to assure you that both antibodies are suitable for your purposes.

They are antibodies g...

Read More

Thank you very much for your interest in ab27775 and ab27776. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. However, I can offer a discount off a future purchase if you buy ab27775 or ab2777...

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Thank you for the clarification. I can see your points now. We do not have a specific category for whole mount staining so I would advise you to ask your customers to select that one which is the closest to the technique and add some special not...

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