VEGF 121B protein (Active) (ab88348)
Constituents: 10% Trehalose, 1% Human serum albumin
Our Abpromise guarantee covers the use of ab88348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Molecular Mass: ab88348 migrates as two bands between 14.5 and 20 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with unmodified VEGF 121 that has a predicted molecular mass of 14 kDa. ab88348 consists of 0-30% carbohydrate by weight.
pI: ab88348 separates into a number of isoforms with a pI between 5.5 and 7.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified VEGF 121 that has a predicted pI of 6.1.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- MVCD1Vascular endothelial growth factor AVascular permeability factor
- VEGFVEGF AVPF
VEGF 121B protein (Active) images
1D SDS-PAGE of ab88348 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab88348
Lane 2: ab88348 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab88348 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. The absence of a drop in MWt after treatment with PNGase F indicates that there are no N-linked glycans attached to the protein. A subsequent drop in MWt after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
A sample of ab88348 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Approximately 40 µg of protein was load. Multiple spot trains indicate the presence of multiple isoforms of ab88348. Spots within both spot trains were cut from the gel and identified as ab88348 by protein mass fingerprinting.
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MWt and pI of the protein.
References for VEGF 121B protein (Active) (ab88348)
ab88348 has not yet been referenced specifically in any publications.