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Overview

  • Product nameVEGF 121B protein (Active)See all VEGF 121B proteins and peptides ...
  • Protein descriptionRecombinant full length Human VEGF 121 expressed in modified human 293 cells; amino acids 27-147 (121aa), Predicted MWt 14kDa (Swiss-Prot P15692).
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityActivity: The ED50 of VEGF 121B is typically 0.5-2.5 ng/ml as measured in a cell proliferation assay using human umbilical vein endothelial (HUVEC) cells.
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsStore at +4°C.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYP DEIEYIFK PSCVPLMRCGGCCNDEGLECVPTEESNITM QIMRIKPHQGQHIGEMSF LQHNKCECRPKKDRARQEKC DKPRR
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab88348 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies
    SDS-PAGE
  • Application notesFuncS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Molecular Mass: ab88348 migrates as two bands between 14.5 and 20 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with unmodified VEGF 121 that has a predicted molecular mass of 14 kDa. ab88348 consists of 0-30% carbohydrate by weight.

    pI: ab88348 separates into a number of isoforms with a pI between 5.5 and 7.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified VEGF 121 that has a predicted pI of 6.1.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        MVCD1Vascular endothelial growth factor AVascular permeability factor
        VEGFVEGF AVPF
      see all
  • RelevanceVEGFA is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. VEGF 121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.
  • Cellular localizationSecreted

    • VEGF 121B protein (Active) images

    • SDS-PAGE - VEGF 121 protein (Active) (ab88348)
      SDS-PAGE - VEGF 121 protein (Active) (ab88348)
      1D SDS-PAGE of ab88348 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1: ab88348
      Lane 2: ab88348 treated with PNGase F to remove potential N-linked glycans
      Lane 3: ab88348 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.

      The absence of a drop in MWt after treatment with PNGase F indicates that there are no N-linked glycans attached to the protein. A subsequent drop in MWt after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
    • SDS-PAGE - VEGF 121 protein (Active) (ab88348)
      SDS-PAGE - VEGF 121 protein (Active) (ab88348)
      A sample of ab88348 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Approximately 40 µg of protein was load. Multiple spot trains indicate the presence of multiple isoforms of ab88348. Spots within both spot trains were cut from the gel and identified as ab88348 by protein mass fingerprinting.
    • Functional Studies - VEGF 121 protein (Active) (ab88348)
      Functional Studies - VEGF 121 protein (Active) (ab88348)
      Densitometry of protein isoforms visualised by 2-DE.
      The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
      The triangle indicates the theoretical MWt and pI of the protein.

    References for VEGF 121B protein (Active) (ab88348)

    ab88348 has not yet been referenced specifically in any publications.

    Publishing research using ab88348 ? Please let us know so that we can cite the reference in this datasheet

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