Anti-VEGF 165A antibody (ab106580)
Key features and details
- Rabbit polyclonal to VEGF 165A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-VEGF 165A antibody
See all VEGF 165A primary antibodies -
Description
Rabbit polyclonal to VEGF 165A -
Host species
Rabbit -
Specificity
ab106580 will recognize both native and recombinant Human VEGF 165A in cell supernatants and certain body fluids. The immunogen is full length recombinant human VEGF-165 protein, but this has high homology with multiple VEGF isoforms, therefore it should recognise these other isoforms too. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human VEGF 165A.
Database link: P15692-4 -
Positive control
- WB: Recombinant human VEGF 165A.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
ab106580 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated. This purified antibody has been heated to 56°C for 30 minutes. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
Target
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Function
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
Tissue specificity
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
Involvement in disease
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Cellular localization
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
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Database links
- Entrez Gene: 7422 Human
- Omim: 192240 Human
- SwissProt: P15692 Human
- Unigene: 73793 Human
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Alternative names
- glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
- MVCD1 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (2)
ab106580 has been referenced in 2 publications.
- Chen H et al. VEGF165 gene-modified human umbilical cord blood mesenchymal stem cells protect against acute liver failure in rats. J Gene Med 23:e3369 (2021). PubMed: 34057770
- Yan Y et al. Modeling of diseases of retinal ischemia in vitro: possible participation of autocrine vascular endothelial growth factor signaling. Ophthalmic Res 49:90-9 (2013). PubMed: 23257772