![Western blot - VEGF 165B antibody [MRVL56/1] (ab14994) image](#ab14994_1.jpg)
![Immunohistochemistry - VEGF 165B antibody [MRVL56/1] (ab14994) image](#ab14994_2.jpg)
![ELISA - VEGF 165B antibody [MRVL56/1] (ab14994) image](#ab14994_3.gif)
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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
Overview
Product name
Anti-VEGF 165B antibody [MRVL56/1]
See all VEGF 165B products (2) ...
Description
Mouse monoclonal [MRVL56/1] to VEGF 165B
Specificity
This antibody has been used to detect VEGF 165b in Western blotting using recombinant protein and protein samples extracted from cells, it specifically detects VEGF 165b but not VEGF 165.
Tested applications
WB, IHC-P, IF, ELISAmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Synthetic peptide (Human) corresponding to the 9 amino acid C-terminal sequence of VEGF165b, coupled to KLH.
Positive control
This antibody gave a positive signal in VEGF 165b HNCO2 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.5
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
MRVL56/1
Isotype
IgG1
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic inhibitory factors
Cancer >> Growth factors >> VEGF
Signal Transduction >> Growth Factors/Hormones >> VEGF
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 23 kDa (predicted molecular weight: 22 kDa).
IHC-P: Use a concentration of 2 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
IF: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
Target
Relevance
Angiogenesis, required for all tumor growth, is stimulated by the expression of vascular endothelial growth factor (VEGF). VEGF is up-regulated in all known solid tumors but also in atherosclerosis, diabetic retinopathy, arthritis, and many other conditions. Conventional VEGF isoforms have been universally described as proangiogenic cytokines. VEGF 165b, an endogenous splice variant, is expressed as protein in normal cells and tissues and is circulating in human plasma. VEGF 165b binds VEGF receptor 2 with the same affinity as VEGF 165 but does not activate it or stimulate downstream signaling pathways. Moreover, it prevents VEGF 165-mediated VEGF receptor 2 phosphorylation and signaling in cultured cells. VEGF 165b is not angiogenic and it inhibits VEGF 165-mediated angiogenesis in rabbit cornea and rat mesentery.
Cellular localization
Secreted
Alternative names
- Vascular Endothelial Growth Factor 165B antibody
- Vascular endothelial growth factor A isoform g antibody
- Vascular permeability factor 165B antibody
see all
Database links
- Entrez Gene: 7422 Human
- Omim: 192240 Human
- SwissProt: P15692-8 Human
Anti-VEGF 165B antibody [MRVL56/1] images:
Western blot - VEGF 165B antibody [MRVL56/1] (ab14994)
![Western blot - VEGF 165B antibody [MRVL56/1] (ab14994)](/ps/datasheet/Images/14/ab14994/ab14994_1.jpg)
Lanes 1 - 2 : clone MRVL56/1 at 1 µg/ml
Lanes 3 - 4 : pan VEGF antibody
Lane 1 : VEGF165B recombinant
Lane 2 : VEGF165 recombinant
Lane 3 : VEGF165B recombinant
Lane 4 : VEGF165 recombinant
Secondary
anti-mouse IgG
developed using the ECL technique
Predicted band size : 22 kDa
Immunohistochemistry - VEGF 165B antibody [MRVL56/1] (ab14994)
![Immunohistochemistry - VEGF 165B antibody [MRVL56/1] (ab14994)](/ps/datasheet/Images/14/ab14994/ab14994_2.jpg)
Top panel: clone MRVL56/1 used on human renal glomerus at 2ug/ml.
Bottom panel: control IgG primary used on human renal glomerus at 2ug/ml.
ELISA - VEGF 165B antibody [MRVL56/1] (ab14994)
![ELISA - VEGF 165B antibody [MRVL56/1] (ab14994)](/ps/datasheet/Images/14/ab14994/ab14994_3.gif)
ELISA on recombinant VEGF 165B.
Blue - clone MRVL56/1
Red - Pan VEGF antibody
Western blot - VEGF 165B antibody [MRVL56/1] (ab14994)
![Western blot - VEGF 165B antibody [MRVL56/1] (ab14994)](/ps/datasheet/images/14/ab14994/VEGF-165B-Primary-antibodies-ab14994-2.jpg)
Anti-VEGF 165B antibody [MRVL56/1] (ab14994) at 5 µg/ml + VEGF 165b HNCO2 at 10 µl
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
Exposure time : 16 minutes
References for Anti-VEGF 165B antibody [MRVL56/1] (ab14994)
This product has been referenced in:
- Baltes-Breitwisch MMet al. Neutralization of vascular endothelial growth factor antiangiogenic isoforms or administration of proangiogenic isoforms stimulates vascular development in the rat testis. Reproduction 140:319-29 (2010). WB; Rat.Read more (PubMed: 20457593) »
- Díaz Ret al. p73 Isoforms affect VEGF, VEGF165b and PEDF expression in human colorectal tumors: VEGF165b downregulation as a marker of poor prognosis. Int J Cancer 123:1060-7 (2008). IHC-P; Human.Read more (PubMed: 18546269) »
See all 3 publications for this product
Publishing research using ab14994? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"