VEGF 165B antibody [MRVL56/1] (ab14994)

Product Name 

VEGF 165B antibody [MRVL56/1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [MRVL56/1] to VEGF 165B

Immunogen 

Synthetic peptide (Human) corresponding to the 9 amino acid C-terminal sequence of VEGF165b, coupled to KLH.

Reacts with 
(species key)

Hu, Rat

Specificity 

This antibody has been used to detect VEGF 165b in Western blotting using recombinant protein and protein samples extracted from cells, it specifically detects VEGF 165b but not VEGF 165.

Tested applications 
(see key)

ELISA, ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic inhibitory factors
Cancer >> Growth factors >> VEGF
Signal Transduction >> Growth Factors/Hormones >> VEGF
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF

Relevance 

Angiogenesis, required for all tumor growth, is stimulated by the expression of vascular endothelial growth factor (VEGF). VEGF is up-regulated in all known solid tumors but also in atherosclerosis, diabetic retinopathy, arthritis, and many other conditions. Conventional VEGF isoforms have been universally described as proangiogenic cytokines. VEGF 165b, an endogenous splice variant, is expressed as protein in normal cells and tissues and is circulating in human plasma. VEGF 165b binds VEGF receptor 2 with the same affinity as VEGF 165 but does not activate it or stimulate downstream signaling pathways. Moreover, it prevents VEGF 165-mediated VEGF receptor 2 phosphorylation and signaling in cultured cells. VEGF 165b is not angiogenic and it inhibits VEGF 165-mediated angiogenesis in rabbit cornea and rat mesentery.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

MRVL56/1

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.5

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for VEGF 165B antibody [MRVL56/1] images, references, products related to ab14994 and other tools.

VEGF 165B antibody [MRVL56/1] images:

References for VEGF 165B antibody [MRVL56/1] (ab14994)

  This product has been used in:

Woolard J et al. VEGF165b, an inhibitory vascular endothelial growth factor splice variant: mechanism of action, in vivo effect on angiogenesis and endogenous protein expression. Cancer Res 64:7822-35 (2004). PubMed: 15520188

If you publish research using ab14994 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding VEGF 165B antibody [MRVL56/1]

Customer FAQs regarding VEGF 165B antibody [MRVL56/1]

Protocols for VEGF 165B antibody [MRVL56/1]

Price and availability of products related to VEGF 165B antibody [MRVL56/1]

VEGF 165B antibody [MRVL56/1] - related products: