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Overview

  • Product nameVEGF Human ELISA KitSee all VEGF kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 10 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 103.5 92% - 113%
    Serum 104.4 92% - 115%
    Plasma 105.7 93% - 114%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • RelevanceVEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated.
    • Alternative names
        MGC70609MVCD1Vascular endothelial growth factor
        Vascular endothelial growth factor AVascular permeability factorVEGF AVEGFAVPF
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100662 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      VEGF Human ELISA Kit images

      • Typical standard curve
        Typical standard curve

        Representative standard curve using ab100662

      • Typical standard curve
        Typical standard curve

        Representative standard curve using ab100662

      Protocols

      References for VEGF Human ELISA Kit (ab100662)

      ab100662 has not yet been referenced specifically in any publications.

      Publishing research using ab100662 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100662 Submit a Question for ab100662

      Displaying 1 - 10 of 11 results for Abreviews and Q&A

      10
      Votes: Highest First

      I would also like to know the epitopes and cross reactivity of the antibody as well as the animal of origin



      Capture Antibody: Monoclonal, mouse. The immunogen sequence is Ala27­Arg191 (Accession # NP_001165097.1) Detects human VEGF165 and human VEGF121. In ELISAs, this antibody has ˜10% cross­reactivity with recombinant mouse VEGF and rat VEGF...

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      Do you know the VEGFA isoform is the antibody raised against?



      The VEGFA isoform that ab100662 detects is 165 and 121.

      Customer requesting clarification about steps in the protocol:
      Question 1:
      1) In the Kit Contents section, it sais to use Diluent A for serum/plasma and Diluent B is for cell culture medium/urine. However, in the Reagent Preparation section step...

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      Answer 1:

      Please use 1X Assay Diluent B to dilute the detection antibody and the HRP-Streptavidin. If testing serum/plasma, use Assay Diluent A to prepare/dilute the standards and samples. Where it says use the “appropriate assay...

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      Product code: 100662
      Lot number: GR65556-1
      Inquiry: I want to do an VEGF Elisa (ab 100662), but can not find in what anti-coagulant I have to collect the plasma in? I also ordered an Elisa kit for PAI1 (ab108891, lot# GR69250-2)and there plasma co...

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      Thank you for contacting us.

      I can confirm that Heparin should not be interfering with the assay using these kits.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information...

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      Dilucion recomendada para muestras de suero.

      Te pido disculpas por la espera.

      He recibido una contestación de mis compañeros, que me comentan que la dilución recomendada para suero y plasma debe hacerse entre 2 y 5 veces.

      En cualquier caso, los niveles de pro...

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      Please check the below and reply me.
      Our customer would like to order as soon as possible.

      Best regards,


      Please note this product is unpublished so when you decide to order you need to contact our customer service team for them to put this item in your order.

      I hope this info will help. Please let me know if you experience any difficulties.

      Our customer purchased 6 different kits and those HRP-streptavidin concentrations are vary.
      Let me know the concentration of this new product and if this is available from now.
      Our customer concerns about the concentration because she has to cha...

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      This product is available but not published on our website. You cannot purchase this using our normal ordering system however I can add this product to your next shipment. Could you confirm how many vials customer would need..6?.
      In my last I made an...

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      Our customer is looking forward to your reply.
      Please check this.
      Best regards

      Thank you for contacting us. I apologize for the delay.

      We are ready to publish this product. The product would be available at US dollar 120 per 200ul. The customer would need 6 vials of product so it will cost them $720 excluding shipping char...

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      Dear technical team,

      Our customer purchased
      ab100586(lot.GR15494),
      ab100662(lot.GR41054),
      ab100606(lot.37486),
      ab100610(lot.GR39199),
      ab100653(lot.GR37277) and
      ab100651(lot.GR37276)
      last year.

      She s...

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      Thank you for your email. I am sorry for the delay while I was in contact with lab.

      The volume of HRP-Streptavidin is very small and therefore would be difficult to see with the naked eye. The HRP-streptavidin volume sent was 8ul and is very con...

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      thank you for your mail, which, however, did not answer my question: why measuring VEGF in cell extracts instead of measuring it in supernatants? to measure also the membrane anchored VEGF? but in this case (ie, protein supposed to increase the levels of ...

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      Thank you for contacting us. We unfortunately are not aware about the purpose of assaying VEGF in cell supernatant or in cell/tissue culture lysates. You may need to search literature to know more about this. The following publication will help to sta...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"