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Overview

  • Product nameVEGF Mouse ELISA KitSee all VEGF kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 2 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.29 83% - 105%
    Serum 87.67 79% - 102%
    Plasma 89.32 81% - 103%

  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab100751 Mouse VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse VEGF in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate Item B 1 x 25ml
    Assay Diluent A . For Standard/Sample (serum/plasma) diluent. 1 x 30ml
    Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium) diluent. 1 x 15ml
    Detection Antibody VEGF: biotinylated anti-mouse VEGF 2 vials
    HRP-Streptavidin Concentrate 160X Item G 1 x 200µl
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
    VEGF Microplate (12 strips x 8 wells) coated with anti-mouse VEGF 1 unit
    VEGF Standard Mouse (recombinant) 2 vials
  • Research Areas
  • RelevanceVEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated.
    • Alternative names
        MGC70609MVCD1Vascular endothelial growth factor
        Vascular endothelial growth factor AVascular permeability factorVEGF AVEGFAVPF
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100751 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      VEGF Mouse ELISA Kit images

      • ELISA - VEGF Mouse ELISA Kit (ab100751)
        ELISA - VEGF Mouse ELISA Kit (ab100751)

      Protocols

      References for VEGF Mouse ELISA Kit (ab100751)

      ab100751 has not yet been referenced specifically in any publications.

      Publishing research using ab100751 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"