VEGF Rat ELISA Kit (ab100787)
- Product nameVEGF Rat ELISA KitSee all VEGF kits ...
- Tests1 x 96 well plate
- Sample typeCell culture extracts, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 2 pg/ml
- Range0.82 pg/ml - 200 pg/ml
Sample specific recovery Sample type Average % Range Cell culture extracts 92.67 81% - 102% Tissue Extracts 94.34 82% - 102%
- Species reactivityReacts with: Rat
- Product overview
ab100787 VEGF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat VEGF cell lysate and tissue lysate. This assay employs an antibody specific for rat VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 2X Cell Lysate Buffer Item J 1 x 5ml Assay Diluent 5x concentrated buffer. For Detection Antibody and HRP-Streptavidin concentrate diluent. 1 x 15ml Detection Antibody VEGF: biotinylated anti-rat VEGF 2 vials HRP-Streptavidin Concentrate 120x concentrated HRP-conjugated streptavidin 1 x 200µl Sample Diluent Buffer 5x concentrated buffer For Standard/Sample (cell lysate/tissue lysate) diluent. 1 x 10ml Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml VEGF Microplate (12 strips x 8 wells) coated with anti-rat VEGF 1 unit VEGF Standard Rat (recombinant) 2 vials
- Research Areas
- MGC70609MVCD1Vascular endothelial growth factor
- Vascular endothelial growth factor AVascular permeability factorVEGF AVEGFAVPF
Our Abpromise guarantee covers the use of ab100787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for VEGF Rat ELISA Kit (ab100787)
ab100787 has not yet been referenced specifically in any publications.