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Product name
VEGF Receptor 1 protein (His tag)
See all VEGF Receptor 1 products (9) ...
Protein description
Recombinant fragment of human VEGF Receptor 1 (aa 783-end) with an N-terminal His tag
Expression host
Baculovirus
Properties
Purification notes
>70% pure by SDS-PAGE
Biological activity
Specific Activity: 170 U/mg. One unit is defined as the amount of enzyme that will transfer 1nmol phosphate to the tyrosine substrate per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP,30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 2 ug /ml recombinant Flt1.
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8
Concentration
Concentration information loading...
Additional notes
This protein was expressed in a Baculovirus infected Sf9 cell expression system.
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Vasculature >> Endothelium
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factors
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Signal Transduction >> Growth Factors/Hormones >> VEGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Inhibition Assay
Application notes
Inhib: Use at an assay dependent dilution. Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
Tissue specificity
Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. It is not expressed in tumor cell lines. Isoform sFlt1 is strongly expressed in placenta.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionP17948
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for VEGF Receptor 1 protein (His tag) (ab42609)
ab42609 has not yet been referenced specifically in any publications.
Publishing research using ab42609? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"