VEGF Receptor 2 Human ELISA Kit (ab100665)
- Product nameVEGF Receptor 2 Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 70 pg/ml
- Range34.3 pg/ml - 25000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 96.23 86% - 104% Serum 96.46 85% - 105% Plasma 94.78 84% - 103%
- Species reactivityReacts with: Human
- Product overview
ab100665 Human VEGF Receptor 2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VEGF Receptor 2 in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human VEGF Receptor 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF Receptor 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human VEGF Receptor 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF Receptor 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
For serum, plasma, cell culture supernatants and urine.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 600X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent A animal serum . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody VEGF R2 : biotinylated anti-human VEGF R2 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml VEGF R2 Microplate (12 strips x 8 wells) coated with anti-human VEGF R2 1 unit VEGF R2 Standard Human (recombinant) 2 vials
- CD309Fetal liver kinase 1FLK1
- KDRKinase insert domain receptorKinase insert domain receptor (a type III receptor tyrosine kinase)Protein tyrosine kinase receptor Flk 1Tyrosine kinase growth factor receptorVascular endothelial growth factor receptor 2VEGFRVEGFR 2VEGFR2
Our Abpromise guarantee covers the use of ab100665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for VEGF Receptor 2 Human ELISA Kit (ab100665)
ab100665 has not yet been referenced specifically in any publications.