Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228)
Key features and details
- Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-VEGF Receptor 2 antibody [mV1001.3m-h]
See all VEGF Receptor 2 primary antibodies -
Description
Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 -
Host species
Mouse -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Purified fragment, corresponding to N terminal amino acids 30-200 of Human VEGF Receptor 2
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Positive control
- Human Umbilical Vein Endothelial Cells (HUVECs).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
mV1001.3m-h -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab42228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGF Receptor 2 (ab11939).
For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab11939 as Detection. |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 151 kDa.
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Notes |
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Sandwich ELISA
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGF Receptor 2 (ab11939). For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab11939 as Detection. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 151 kDa. |
Target
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Function
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Involvement in disease
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3791 Human
- Omim: 191306 Human
- SwissProt: P35968 Human
- Unigene: 479756 Human
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Alternative names
- CD309 antibody
- CD309 antigen antibody
- EC 2.7.10.1 antibody
see all
Images
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Lane 1 : unstimulated cells
Lane 2 : hVEGF at 20 ng/ml
Lane 3 : Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228) at 5 µg/ml
Lane 4 : Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228) at 25 µg/ml
All lanes : HUVECs
Predicted band size: 151 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
HUVECs (human umbilical vein endothelial cells) were stimulated with hVEGF or ab42228 for 30min. Phospho-VEGF Receptor 2 was detected with IP-Western for Phosphotyrosine.
Datasheets and documents
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Datasheet download
References (4)
ab42228 has been referenced in 4 publications.
- Silva JAF et al. Spatial and temporal VEGF receptor intracellular trafficking in microvascular and macrovascular endothelial cells. Sci Rep 11:17400 (2021). PubMed: 34462507
- Peng F et al. PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis. Int J Oncol 52:945-954 (2018). PubMed: 29393396
- Liu H et al. Human umbilical vein endothelial cell vaccine suppresses the angiogenesis of esophageal squamous cell carcinoma in a humanized mouse model. Oncol Rep 40:3006-3014 (2018). PubMed: 30132560
- Ptaszynska MM et al. Positive Feedback between Vascular Endothelial Growth Factor-A and Autotaxin in Ovarian Cancer Cells. Mol Cancer Res 6:352-63 (2008). PubMed: 18337445