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- Secondary antibodies
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Product name
Anti-VEGF Receptor 2 (phospho Y951) antibody
See all VEGF Receptor 2 products (36) ...
Description
Rabbit polyclonal to VEGF Receptor 2 (phospho Y951)
Specificity
This antibody is specific for VEGF Receptor 2 only when phosphorylated at tyrosine 951.
Tested applications
WB, ELISA, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic phosphopeptide derived from Human VEGF Receptor 2 around the phosphorylation site of tyrosine 951.
Positive control
Breast carcinoma tissue and SK-OV3 cell extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Cardiovascular >> Cardiovascular Markers >> Cell Markers >> Endothelial Cells
Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Signal Transduction >> Growth Factors/Hormones >> VEGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 151 kDa).
ELISA
ELISA: 1/20000.ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
ICC/IF: 1/100.
Target
Function
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Involvement in disease
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
Cellular localization
Membrane.
Target information above from: UniProt accessionP35968
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD309 antibody
- CD309 antigen antibody
- Fetal liver kinase 1 antibody
see all
Database links
- Entrez Gene: 3791 Human
- Entrez Gene: 16542 Mouse
- Entrez Gene: 25589 Rat
- Omim: 191306 Human
- SwissProt: P35968 Human
- SwissProt: P35918 Mouse
- SwissProt: O08775 Rat
- Unigene: 479756 Human
see all
Anti-VEGF Receptor 2 (phospho Y951) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - VEGF Receptor 2 (phospho Y951) antibody (ab38473)
ab38473 at a 1:50 dilution staining VEGF Receptor 2 in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.
Left image : untreated.
Right image : treated with phosphopeptide.
Western blot - VEGF Receptor 2 (phospho Y951) antibody (ab38473)
All lanes : Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473) at 1/500 dilution
Lane 1 : SK-OV3 cell extract (5-30ug of total protein).
Lane 2 : SK-OV3 cell extract (5-30ug of total protein). Treated with phosphopeptide.
Secondary
Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)
Predicted band size : 151 kDa
Observed band size : 190 kDa (why is the actual band size different from the predicted?)
References for Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)
This product has been referenced in:
- Sangaletti S et al. Macrophage-derived SPARC bridges tumor cell-extracellular matrix interactions toward metastasis. Cancer Res 68:9050-9 (2008). IHC-P; Mouse.Read more (PubMed: 18974151) »
See 1 publication for this product
Publishing research using ab38473? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"