Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)
Overview
- Product nameAnti-VEGF Receptor 2 (phospho Y951) antibodySee all VEGF Receptor 2 primary antibodies ...
- DescriptionRabbit polyclonal to VEGF Receptor 2 (phospho Y951)
- SpecificityThis antibody is specific for VEGF Receptor 2 only when phosphorylated at tyrosine 951.
- Tested applicationsWB, ELISA, IHC-P, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat - Immunogen
Synthetic phosphopeptide derived from Human VEGF Receptor 2 around the phosphorylation site of tyrosine 951.
- Positive controlBreast carcinoma tissue and SK-OV3 cell extract.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab38473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 151 kDa). |
| ELISA | ELISA: 1/20000. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| ICC/IF | ICC/IF: 1/100. |
Target
- FunctionReceptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
- Involvement in diseaseDefects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
- Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. - Post-translational
modificationsPhosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. - Cellular localizationMembrane.
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Database links
- Entrez Gene: 3791 Human
- Entrez Gene: 16542 Mouse
- Entrez Gene: 25589 Rat
- Omim: 191306 Human
- SwissProt: P35968 Human
- SwissProt: P35918 Mouse
- SwissProt: O08775 Rat
- Unigene: 479756 Human
- Unigene: 285 Mouse
- Unigene: 88869 Rat
see all
Target information above from: UniProt accession
P35968
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CD309 antibodyCD309 antigen antibodyFetal liver kinase 1 antibody
- FLK-1 antibodyFLK1 antibodyKDR antibodyKinase insert domain receptor (a type III receptor tyrosine kinase) antibodyKinase insert domain receptor antibodyKRD1 antibodyLy73 antibodyProtein tyrosine kinase receptor FLK1 antibodyProtein-tyrosine kinase receptor flk-1 antibodyTyrosine kinase growth factor receptor antibodyVascular endothelial growth factor receptor 2 antibodyVEGFR 2 antibodyVEGFR antibodyVEGFR-2 antibodyVEGFR2 antibodyVGFR2_HUMAN antibody
see all
Anti-VEGF Receptor 2 (phospho Y951) antibody images
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Immunohistochemistry (Paraffin-embedded sections) - VEGF Receptor 2 (phospho Y951) antibody (ab38473)ab38473 at a 1:50 dilution staining VEGF Receptor 2 in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.Left image : untreated.
Right image : treated with phosphopeptide.
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Western blot - VEGF Receptor 2 (phospho Y951) antibody (ab38473)All lanes : Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473) at 1/500 dilution
Lane 1 : SK-OV3 cell extract (5-30ug of total protein).
Lane 2 : SK-OV3 cell extract (5-30ug of total protein). Treated with phosphopeptide.
Secondary
Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)
Predicted band size : 151 kDa
Observed band size : 190 kDa (why is the actual band size different from the predicted?)
References for Anti-VEGF Receptor 2 (phospho Y951) antibody (ab38473)
This product has been referenced in:
- Sangaletti S et al. Macrophage-derived SPARC bridges tumor cell-extracellular matrix interactions toward metastasis. Cancer Res 68:9050-9 (2008). IHC-P ; Mouse . Read more (PubMed: 18974151) »
