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Anti-VEGF Receptor 2 (phospho Y951) antibody (ab5464)

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Overview

  • Product nameAnti-VEGF Receptor 2 (phospho Y951) antibodySee all VEGF Receptor 2 primary antibodies ...
  • Description
    Rabbit polyclonal to VEGF Receptor 2 (phospho Y951)
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phospho peptide (Human) containing Tyrosine 951.

  • Positive controlNIH3T3 cells transfected with full-length, wild-type human VEGF Receptor 2.

Properties

Applications

Our Abpromise guarantee covers the use of ab5464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 200 kDa.

Target

  • FunctionReceptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in diseaseDefects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P35968 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        CD309 antibodyCD309 antigen antibodyFetal liver kinase 1 antibody
        FLK-1 antibodyFLK1 antibodyKDR antibodyKinase insert domain receptor (a type III receptor tyrosine kinase) antibodyKinase insert domain receptor antibodyKRD1 antibodyLy73 antibodyProtein tyrosine kinase receptor FLK1 antibodyProtein-tyrosine kinase receptor flk-1 antibodyTyrosine kinase growth factor receptor antibodyVascular endothelial growth factor receptor 2 antibodyVEGFR 2 antibodyVEGFR antibodyVEGFR-2 antibodyVEGFR2 antibodyVGFR2_HUMAN antibody
      see all

    Anti-VEGF Receptor 2 (phospho Y951) antibody images

    • Western blot - Anti-VEGF Receptor 2 (phospho Y951) antibody (ab5464)
      Western blot - Anti-VEGF Receptor 2 (phospho Y951) antibody (ab5464)
      Peptide Competition and Stimulation: Extracts prepared from NIH3T3 cells transfected with human VEGFR2 and left unstimulated (1) or stimulated with VEGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA TBST buffer overnight at 4°C, then were incubated with 0.40 µg/mL ab5464 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phospho-tyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that the signal is strongly induced by VEGF treatment and that only the peptide corresponding to ab5464 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

    References for Anti-VEGF Receptor 2 (phospho Y951) antibody (ab5464)

    ab5464 has not yet been referenced specifically in any publications.

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