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Product name
VEGF Receptor 2 protein (Tagged)
See all VEGF Receptor 2 products (5) ...
Protein description
Recombinant fragment, corresponding to amino acids 805-1356 of Human VEGF Receptor 2, with an N terminal proprietary tag.
Expression host
Baculovirus
Properties
Purity
> 90% by SDS-PAGE
Biological activity
40 U/µg. One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na-orthovanadate, 0.5 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate and 1 µg/ml recombinant VEGF Receptor 2.
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, 10mM Reduced glutathione, pH 8
Concentration
Concentration information loading...
Additional notes
This protein was expressed using a Baculovirus infected Sf9 cell expression system.
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Cardiovascular >> Cardiovascular Markers >> Cell Markers >> Endothelial Cells
Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Signal Transduction >> Growth Factors/Hormones >> VEGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Inhibition Assay
Application notes
Inhib: Use at an assay dependent dilution. Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Involvement in disease
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
Cellular localization
Membrane.
Target information above from: UniProt accessionP35968
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for VEGF Receptor 2 protein (Tagged) (ab42614)
ab42614 has not yet been referenced specifically in any publications.
Publishing research using ab42614? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"