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VEGF Receptor 2 protein (Tagged) (ab42614)

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Overview

  • Product nameVEGF Receptor 2 protein (Tagged)See all VEGF Receptor 2 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 805-1356 of Human VEGF Receptor 2, with an N terminal proprietary tag.
  • Expression hostBaculovirus

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Biological activity40 U/µg. One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na-orthovanadate, 0.5 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate and 1 µg/ml recombinant VEGF Receptor 2.
  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, 10mM Reduced glutathione, pH 8
  • Concentration information loading...
  • Additional notesThis protein was expressed using a Baculovirus infected Sf9 cell expression system.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab42614 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Inhibition Assay
  • Application notesInhib: Use at an assay dependent dilution. Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CD309CD309 antigenFetal liver kinase 1
        FLK-1FLK1KdrKinase insert domain receptorKinase insert domain receptor (a type III receptor tyrosine kinase)KRD1Ly73Protein tyrosine kinase receptor FLK1Protein-tyrosine kinase receptor flk-1Tyrosine kinase growth factor receptorVascular endothelial growth factor receptor 2VEGFRVEGFR 2VEGFR-2VEGFR2VGFR2_HUMAN
      see all
  • FunctionReceptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in diseaseDefects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P35968 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for VEGF Receptor 2 protein (Tagged) (ab42614)

    ab42614 has not yet been referenced specifically in any publications.

    Publishing research using ab42614 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab42614 Submit a Question for ab42614

    Displaying 1 - 6 of 6 results for Abreviews and Q&A

    10
    Votes: Highest First

    If the notes refer to the full length of protein, what the phosphorylation status of this product ab42614?

    I am sorry my previous email was unclear.

    The phosphorylation status of ab42614 is no known. This is not something that is determined during the production process.

    I hope this information is helpful.



    Thank you very much for your great suggestion. This product is perfect except the Agarose beads. Because we will use the centrifuge to separate the particle, the

    rigidity ofAgarose may be not enough. However, I got very useful inform...

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    Thank you for your reply.

    Actually it is fine to centrifuge agarose beads. Typically for IP I have centrifuged agarose beads at 500xg for 3-5 min at 4 degrees C.

    Regarding your questions 1 and 2, those notes on the datasheet for ab4261...

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    Sorry to interrupt you again. I really got some further questions.



    Actually, I want to choose an Anti-GST antibody as a “bait” to fish out the GST-tagged VEGFR. For the bait, I hope to immobilize the antibody to some par...

    Read More

    Thank you for your enquiry.

    I think I have found a product that will suit your needs:

    http://www.abcam.com/GST-antibody-Agarose-ab90667.html

    This is anti-GST antibody covalently linked to agarose beads. Using this product wou...

    Read More


    Thanks a lot for your useful information. I appreciate it very much.

    I'm happy to help! Please contact me again if you need anything further.

    Hi,



    I’m sorry to interrupt you. I have some questions about your product.



    We just bought a VEGF Receptor 2 protein (Tagged) (ab42614) from your company. However, we can’t find the Tag information f...

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    Thank you for your enquiry.

    VEGF Receptor 2 protein (Tagged) (ab42614) contains an N-terminal GST tag.

    I hope this is helpful. Please contact me again if you have any further questions.

    I am interested in VEGF recepotr 2 protein (ab54347). I want to use it in a kinase assay. So I'd like to know whether this product has kinase activity?  

    Thank you for your patience while I looked into your question. I have heard back from the lab, and ab106310 is also only the extracellular domain of VEGF receptor 2, and is thus not suitable for kinase studies. However, ab42614 has been tested for...

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