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VEGF Receptor 2 protein (Active) (ab54347)

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Overview

  • Product nameVEGF Receptor 2 protein (Active)See all VEGF Receptor 2 proteins and peptides ...
  • Protein descriptionRecombinant fragment from Human VEGFR2; M.Wt 116 kDa. Glycosylated monomer. This protein consists of all 7 extracellular domains, which contain all the information necessary for high affinity ligand binding. Source: Insect cells.
  • Expression hostInsect cells

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notesab54347 was visualised on SDS Page by Silver stain. Endotoxin level: < 0.1 ng per ug of sKDR
  • FormLyophilised:Reconstitute in water or PBS to a concentration of not lower than 100 µg/ml.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 100mM Sodium chloride, 10mM Tris, pH 8.0
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab54347 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent dilution. Abolishes the binding of iodinated VEGF to solid surfaces or cell surface receptors with a typical ED50 of 10 ng/ml.

    Protein info

    • Alternative names
        CD309CD309 antigenFetal liver kinase 1
        FLK-1FLK1KDRKinase insert domain receptorKinase insert domain receptor (a type III receptor tyrosine kinase)KRD1Ly73Protein tyrosine kinase receptor FLK1Protein-tyrosine kinase receptor flk-1Tyrosine kinase growth factor receptorVascular endothelial growth factor receptor 2VEGFRVEGFR 2VEGFR-2VEGFR2VGFR2_HUMAN
      see all
  • FunctionReceptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in diseaseDefects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P35968 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    VEGF Receptor 2 protein (Active) images

    References for VEGF Receptor 2 protein (Active) (ab54347)

    ab54347 has not yet been referenced specifically in any publications.

    Publishing research using ab54347 ? Please let us know so that we can cite the reference in this datasheet

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    Dear Tech support

    Attached is a customer complaint form for ab106041.
    Here is what the customer has written in addition:



    I tested the VEGF receptors (ab54346, ab106310 & ab54347) with the Rabbit anti-VEGF-HRP (ab106041)...

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    Thank you for your enquiry regarding ab106041 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    After reading through the detailed protocol you kind...

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    Is there a recomended shelf life for Abcam primary antibodies under proper storage conditions (as writen in the datasheet)?
    Could you please specify the shelf life for the following products:
    ab106041
    ab106310
    ab51516
    ab54346
    a...

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    Thank you for contacting us.

    The antibodies are viable for 10 years when stored at -20C, for 1 year when stored at 4C.

    Please note that we recommend storing the antibodies at 4C for 1-2 weeks only. Some antibodies are prone to degradatio...

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    Called to inquire about obtaining standards and diluents for VEGF R2 kit.

    Thank you for contacting Abcam regarding this.

    Unfortunately because this information is considered proprietary, I am not able to share the exact contents of the standard/sample diluent other than to tell you it is an animal serum with 0.09%sod...

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    I am interested in VEGF recepotr 2 protein (ab54347). I want to use it in a kinase assay. So I'd like to know whether this product has kinase activity?  

    Thank you for your patience while I looked into your question. I have heard back from the lab, and ab106310 is also only the extracellular domain of VEGF receptor 2, and is thus not suitable for kinase studies. However, ab42614 has been tested for...

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    I am interested in VEGF recepotr 2 protein (ab54347). I want to use it in a kinase assay. So I'd like to know whether this product has kinase activity?  

    Thank you for contacting us with your question. Ab54347 is composed of the 7 extracellular ligand binding domains, and it does not contain the intracellular tyrosine kinase domain. So, this protein would not be suitable for kinase assays. We have an...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"