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Serum-starved porcine aortic endothelial cells were incubated with recombinant VEGF proteins, and the phosphorylation status of VEGFRs or Akt was analyzed from cell lysates.The effect of VEGF-D?N?C and C25L on the phosphorylation of VEGFR-3 in PAE-Flt4 cells at 5, 15, and 30 minute time points. Cell lysates were immunoprecipitated using ab10284 and analyzed by immunoblotting with an anti phosphotyrosine antibody. Equal loading was confirmed using ab10284.
Image from Toivanen PI et al, J Biol Chem. 2009 Jun 5;284(23):16037-48. Epub 2009 Apr 14, Fig 7. DOI 10.1074/jbc.M109.001123
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