Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
Overview
Product name
Anti-VEGF Receptor 3 antibody
See all VEGF Receptor 3 products (10) ...
Description
Rabbit polyclonal to VEGF Receptor 3
Tested applications
ELISA, WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant human soluble VEGFR3/FLT4 protein (110 kDa)(D1-D7).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Signal Transduction >> Growth Factors/Hormones >> VEGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
-
Immunoprecipitation - VEGF Receptor 3 antibody (ab10284)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
- 1 Image
IP
Application notes
ELISA: Use at a concentration of 0.5 - 1.5 µg/ml.
IP: Use at a concentration of 1 - 5 µg/ml of lysate.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 164 kDa.
In Western blots, this antibody shows a moderate cross-reactivity with VEGFR2/KDR and a weak cross-reactivity with VEGFR1/FLT1.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Receptor for VEGFC. Has a tyrosine-protein kinase activity.
Tissue specificity
Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.
Involvement in disease
Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionP35916
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 2.7.10.1 antibody
- flt 4 antibody
- FLT-4 antibody
see all
Database links
- Entrez Gene: 2324 Human
- Omim: 136352 Human
- SwissProt: P35916 Human
- Unigene: 646917 Human
Anti-VEGF Receptor 3 antibody images:
Immunoprecipitation - VEGF Receptor 3 antibody (ab10284)
Serum-starved porcine aortic endothelial cells were incubated with recombinant VEGF proteins, and the phosphorylation status of VEGFRs or Akt was analyzed from cell lysates.The effect of VEGF-D?N?C and C25L on the phosphorylation of VEGFR-3 in PAE-Flt4 cells at 5, 15, and 30 minute time points. Cell lysates were immunoprecipitated using ab10284 and analyzed by immunoblotting with an anti phosphotyrosine antibody. Equal loading was confirmed using ab10284.
Image from Toivanen PI et al, J Biol Chem. 2009 Jun 5;284(23):16037-48. Epub 2009 Apr 14, Fig 7. DOI 10.1074/jbc.M109.001123
References for Anti-VEGF Receptor 3 antibody (ab10284)
This product has been referenced in:
- Toivanen PIet al. Novel vascular endothelial growth factor D variants with increased biological activity. J Biol Chem 284:16037-48 (2009). IP; Pig.Read more (PubMed: 19366703) »
See 1 publication for this product
Publishing research using ab10284? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"