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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
Overview
Product name
Anti-VEGF Receptor 3 antibody
See all VEGF Receptor 3 products (10) ...
Description
Rabbit polyclonal to VEGF Receptor 3
Tested applications
ICC/IF, WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: LASEEFEQIESRHRQESGFR, corresponding to C terminal amino acids 1279-1298 of Human VEGF Receptor 3
LASEEFEQIE SRHRQESGFR
Positive control
Human placenta or breast carcinoma for Immunohistochemistry.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Other
Signal Transduction >> Growth Factors/Hormones >> VEGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: Use at an assay dependent dilution. Predicted molecular weight: 146 kDa.
IP: Use at an assay dependent dilution.
IHC-P: 1/100Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
Receptor for VEGFC. Has a tyrosine-protein kinase activity.
Tissue specificity
Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.
Involvement in disease
Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionP35916
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Chy antibody
- EC 2.7.10.1 antibody
- flt 4 antibody
see all
Database links
- Entrez Gene: 2324 Human
- Entrez Gene: 14257 Mouse
- Entrez Gene: 114110 Rat
- Omim: 136352 Human
- SwissProt: P35916 Human
- SwissProt: P35917 Mouse
- SwissProt: Q91ZT1 Rat
- Unigene: 646917 Human
see all
Anti-VEGF Receptor 3 antibody images:
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Flt4 antibody (ab27278)
Ab27278 at a dilution of 1/100, staining formalin fixed paraffin embedded Flt4 in human placenta by Immunohistochemistry.
Western blot - Flt4 antibody (ab27278)
Anti-VEGF Receptor 3 antibody (ab27278) at 1/50 dilution
Predicted band size : 146 kDa
Observed band size : 170,200 kDa (why is the actual band size different from the predicted?)
Hey cell lysate (50µg/lane) was used. The antibody was diluted at 1:50 for 2 hours at room temperature. The protein is heavily glycosylated and this might explain the increase in MW above the predcted MW as well as the multiple bands.
Immunocytochemistry/ Immunofluorescence - VEGF Receptor 3 antibody (ab27278)
ICC/IF image of ab27278 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27278, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-VEGF Receptor 3 antibody (ab27278)
This product has been referenced in:
- Hernández-Miranda LRet al. Robo1 regulates semaphorin signaling to guide the migration of cortical interneurons through the ventral forebrain. J Neurosci 31:6174-87 (2011).Read more (PubMed: 21508241) »
- Cooley LSet al. Reversible transdifferentiation of blood vascular endothelial cells to a lymphatic-like phenotype in vitro. J Cell Sci 123:3808-16 (2010). WB, ICC/IF; Human.Read more (PubMed: 20940254) »
See all 3 publications for this product
Publishing research using ab27278? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"