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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VEGF antibody [5C3.F8] (ab3109)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VEGF antibody [5C3.F8] (ab3109)](/ps/datasheet/images/ab3109_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VEGF antibody [5C3.F8] (ab3109)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VEGF antibody [5C3.F8] (ab3109)](/ps/datasheet/images/ab3109_2.jpg)
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Product Name
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VEGF antibody [5C3.F8]
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See all VEGF antibodies (25)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [5C3.F8] to VEGF
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Immunogen
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Human VEGF from a glioma.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/50.
WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT (reducing conditions only).
Detects a band of approximately 19-22 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: human VEGF 165 recombinant protein
IHC-P: mouse islet grafts
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Cellular localization
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Cytoplasmic and Secreted
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Angiogenesis and vasculogenesis
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Signal Transduction >> Growth Factors/Hormones >> VEGF
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
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Relevance
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VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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5C3.F8
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VEGF antibody [5C3.F8] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VEGF antibody [5C3.F8] images, references, products related to ab3109 and other tools.
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VEGF antibody [5C3.F8] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VEGF antibody [5C3.F8] (ab3109)
ab3109 staining VEGF in mouse islet grafts by Immunohistochemistry (FFPE-sections). Copyright American Diabetes Association from Diabetes Vol 53, 2004:963-970. Reprinted with permission from the American Diabetes Association
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VEGF antibody [5C3.F8] (ab3109)
ab3109 staining VEGF in mouse islet grafts by Immunohistochemistry (FFPE-sections). Copyright American Diabetes Association from Diabetes Vol. 53, 2004:963-970. Reprinted with permission from the American Diabetes Association
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References for VEGF antibody [5C3.F8] (ab3109)
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This product has been used in: (two most recent references)
Puglisi F et al. Expression of periostin in human breast cancer. J Clin Pathol 61:494-8 (2008). PubMed: 17938160
Adler AS et al. CSN5 isopeptidase activity links COP9 signalosome activation to breast cancer progression. Cancer Res 68:506-15 (2008). IHC-P; Mouse. PubMed: 18199546
See all 4 publication references for this product.
If you publish research using ab3109 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to VEGF
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VEGF antibody [5C3.F8] - more information
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Customer reviews (feedback) regarding VEGF antibody [5C3.F8] |
Customer FAQs regarding VEGF antibody [5C3.F8] |
Protocols for VEGF antibody [5C3.F8] |
Price and availability of products related to VEGF antibody [5C3.F8] |
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VEGF antibody [5C3.F8] - related products:
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VEGF antibody [5C3.F8] - other tools:
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Contact Abcam with a Technical Enquiry about VEGF antibody [5C3.F8] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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