Anti-VEGF antibody [VG76e] (ab119)

Yes, I've looked up the Lab Vision Corporation and ordered another antibody for VEGF from them.

If Vg76e can be used on native or denatured forms, then the antibody you supplied us with is not working. We've spend alot time trying to get it working and tried many experimental conditions, so you may want to note that for your records. You may also want to check the same batch or even my supply of it yourselves.

ANSWER:

Thank you for getting back in touch with me. Im afraid that I have provided you with all of the information that I have with regards this antibody. Given the information that you have obtained it is highly plausible that the non-reducing conditions are necessary for the detection of VEGF using this clone.

I will certainly endevour to note this on the datasheet.

The company is called Lab Vision Corporation and this is the link to the page that compares different VEGF antibodies (VG76e is the 3rd one down):

http://www.labvision.com/ab1.cfm?First=AntiBody&Second=Vascular%20Endothelial%20Growth%20Factor

There is also a data sheet for the antibody, which is useful too:

http://www.labvision.com/pdf/999.pdf

They have a picture of the band on a non-reducing gel. Is it necessary to use a non-denaturing gel or is it sufficient to leave out adding the reducing agent, like B-mercpatoethanol?

Another question: do you know of any published literature which has used this antobody for western blotting. If so, could you send me a link to that?

ANSWER:

Thank you for getting back to me.

I am currently in touch with the lab to determine whether they have any further information as to the analysis of VEGF using a non-reducing gel. Yes, the omission of beta-mercaptoethanol would be sufficient for this kind of analysis.

I will be back in touch when I have further information.

I hope you got the questionnaire I filled out...thank you for sending that. I found some information from another company which said that this clone of antibody (VG76e) should only be used for un-reduced VEGF. Is it possible that this antibody only binds to an epitope that is exposed on the dimer? If so, could you suggest a protocol?

Thank you and I look forward to hearing from you.

ANSWER:

Thank you for getting back to me.

That is certainly a possibility. The clones should be identical aside from individual laboratory preparation differences. Please can you tell me where you obtained this information for this clone, I am most interested in confirming the epitope information for this antibody.

I am interested in purchasing Abcam.s VEGF antibody. Does this antibody recognise all four isoforms of VEGF, or does it only recognise VEGF 189?

ANSWER:

Thank you for your enquiry. To our knowledge this product will cross react with VEGF165 ( J Pathol, 185: 402-408 (1998). We do not have any information regarding the product's cross reactively with other isoforms of VEGF. If you need further information, please contact us again and we will gladly assist you.

Can you please ley me know the dilution for the antibody ab119 use in IHC. Which of these two item are more commonly be used?

Thanks. Best Regards.

ANSWER:

Thanks for your email. I'm replying to you on behalf of Chris, who is currently away on vacation. To use ab119 in IHC, I would recommend trying a range of dilutions in order to determine which is optimal for your particular experiment: 1:50, 1:100, 1:200. We do sell more of ab119 compared to ab3109. If you have any more questions, please let me know.