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Western blot - VEGF antibody (ab46154)
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Immunocytochemistry/ Immunofluorescence - VEGF antibody (ab46154)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VEGF antibody (ab46154)
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Product Name
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VEGF antibody
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See all VEGF antibodies (25)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to VEGF
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human VEGF. (Peptides available as ab46160).
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at an assay dependent concentration.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 24, 45 kDa). Can be blocked with VEGF peptide (ab46160).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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ab46154 gave a positive result in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line)
Jurkat (Human T cell lymphoblast-like cell line)
A431 (Human epithelial carcinoma cell line)
HEK 293 (Human embryonic kidney cell line)
HepG2 (Human hepatocellular liver carcinoma cell line)
MCF7 (Human breast adenocarcinoma cell line)
SHSY-5Y (Human neuroblastoma cell line)
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Cellular localization
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Cytoplasmic and Secreted
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Angiogenesis and vasculogenesis
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Signal Transduction >> Growth Factors/Hormones >> VEGF
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
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Relevance
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VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.20 - 1.00 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VEGF antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VEGF antibody images, references, products related to ab46154 and other tools.
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VEGF antibody images:
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 Immunocytochemistry/ Immunofluorescence - VEGF antibody (ab46154)
ICC/IF image of ab46154 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab46154, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VEGF antibody (ab46154)
ab46154 staining kidney tissue sections (human, mouse and rat) by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval prior to blocking with sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. This image is courtesy of an Abreview submitted by Antibody Solutions Ltd
See Abreview
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References for VEGF antibody (ab46154)
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This product has been used in: (two most recent references)
Pifarr P et al. Cyclic GMP phosphodiesterase inhibition alters the glial inflammatory response, reduces oxidative stress and cell death and increases angiogenesis following focal brain injury. J Neurochem 112:807-17 (2010). IHC-P; Rat. PubMed: 20002517
Li M et al. Phospholipase Cepsilon promotes intestinal tumorigenesis of Apc(Min/+) mice through augmentation of inflammation and angiogenesis. Carcinogenesis 30:1424-32 (2009). PubMed: 19458037
If you publish research using ab46154 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to VEGF
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VEGF antibody - more information
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Customer reviews (feedback) regarding VEGF antibody |
Customer FAQs regarding VEGF antibody |
Protocols for VEGF antibody |
Price and availability of products related to VEGF antibody |
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VEGF antibody - related products:
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VEGF antibody - other tools:
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Contact Abcam with a Technical Enquiry about VEGF antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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