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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VEGF antibody (ab9570)
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Product Name
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VEGF antibody
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See all VEGF antibodies (25)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to VEGF
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Immunogen
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Highly pure (>98%) recombinant hVEGF 165 (human Vascular Endothelial Cell Growth Factor)
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Reacts with
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Hu
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Specificity
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Reactive with VEGF 121 as well as VEGF 165.
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Tested applications
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ELISA, IHC-P, Neut, WB
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Abreviews
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Application notes
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Recommended dilutions
IHC-P: Use at a concentration of 2.5 - 5.5 µg/ml.
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hVEGF (10.0 ng/ml), a concentration of 0.05 - 0.1 µg/ml of this antibody is required.
ELISA:To detect hVEGF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hVEGF.
WB: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic and Secreted
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Angiogenesis and vasculogenesis
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Signal Transduction >> Growth Factors/Hormones >> VEGF
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
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Relevance
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VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS, pH 7.4, no preservative, sterile filtered
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VEGF antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VEGF antibody images, references, products related to ab9570 and other tools.
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VEGF antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VEGF antibody (ab9570)
ab9570 staining VEGF in human breast invasive ductal carcinoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 2.5 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
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Search PubMed (MEDLINE) for references to VEGF
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VEGF antibody - more information
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Customer reviews (feedback) regarding VEGF antibody |
Customer FAQs regarding VEGF antibody |
Protocols for VEGF antibody |
Price and availability of products related to VEGF antibody |
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VEGF antibody - related products:
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VEGF antibody - other tools:
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Contact Abcam with a Technical Enquiry about VEGF antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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