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Anti-VEGFA antibody (ab51745)

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1 question for ab51745

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Question 1

Friday 25-July-2008

I have a question about ab51745 This antibody's immunogen is Recombinant murine VEGF164 Dimer. But predicted size is 27KDa. This size is monomer. Our user wants to detect just monomer. Could you confirm which one is detecting?

ANSWER:

  

Thank you for your enquiry.

The MW of recombinant mouse VEGF164 dimer produced in insect cells is about 48 kDa, but the monomer has a MW of about 24 kDa.. The predicted size of 27 kDa is probably those of VEGF164 produced in mammalian cells. This is because insect cells have a different glycosylation than mammalian cells. The glycosylation in insect cells is lower than those of mammalian cells. If your customer wants to detect the monomer, he has to run the SDS-PAGE under reducing conditions.

I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

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