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Product Name
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VEGFB antibody
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See all VEGFB antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to VEGFB
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Immunogen
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Recombinant full length VEGFB 186 (Human), expressed in E. coli.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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Anti-human VEGFB exhibits less than 5% cross-reactivity with recombinant human VEGFD and no cross-reactivity with recombinant human VEGF and recombinant mouse VEGF.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant human VEGFB 167 is approximately 0.3 ng/well. The detection limit for recombinant human VEGFB 186 is approximately 0.03 ng/well.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human VEGFB 167 is approximately 25 ng/lane under non-reducing and reducing conditions. The detection limit for recombinant human VEGFB 186 is approximately 5 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 22 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
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Research areas
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Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> VEGF
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
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Relevance
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Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 15mM sodium azide.
Constituents: 0.01M PBS, 1% BSA. pH 7.4
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Purification notes
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VEGFB 167/186 specific IgG is purified by passing the goat sera over a human VEGFB 186 affinity column. The bound fraction is then purified on a human VEGFB 167 affinity column.
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Do not store in a frost-free freezer.
Endotoxin level is < 30 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VEGFB antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to VEGFB
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VEGFB antibody - more information
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Customer reviews (feedback) regarding VEGFB antibody |
Customer FAQs regarding VEGFB antibody |
Protocols for VEGFB antibody |
Price and availability of products related to VEGFB antibody |
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VEGFB antibody - related products:
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Contact Abcam with a Technical Enquiry about VEGFB antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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