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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
Overview
Product name
Anti-VEGFB antibody
See all VEGFB products (6) ...
Description
Goat polyclonal to VEGFB
Specificity
Anti-human VEGFB exhibits less than 5% cross-reactivity with recombinant human VEGFD and no cross-reactivity with recombinant human VEGF and recombinant mouse VEGF.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant full length VEGFB 186 (Human), expressed in E. coli.
General notes
Do not store in a frost-free freezer.
Endotoxin level is < 30 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM sodium azide.
Constituents: 0.01M PBS, 1% BSA. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
VEGFB 167/186 specific IgG is purified by passing the goat sera over a human VEGFB 186 affinity column. The bound fraction is then purified on a human VEGFB 167 affinity column.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> VEGF
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
Application notes
ELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant human VEGFB 167 is approximately 0.3 ng/well. The detection limit for recombinant human VEGFB 186 is approximately 0.03 ng/well.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human VEGFB 167 is approximately 25 ng/lane under non-reducing and reducing conditions. The detection limit for recombinant human VEGFB 186 is approximately 5 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 22 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
Tissue specificity
Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
Sequence similarities
Belongs to the PDGF/VEGF growth factor family.
Post-translational
modifications
VEGF-B186 is O-glycosylated.
Cellular localization
Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
Target information above from: UniProt accessionP49765
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
There are 2 isoforms produced by alternative splicing.
Alternative names
- vascular endothelial growth factor B antibody
- Vascular endothelial growth factor B precursor antibody
- VEGF B antibody
see all
Database links
- Entrez Gene: 7423 Human
- Entrez Gene: 22340 Mouse
- Omim: 601398 Human
- SwissProt: P49765 Human
- SwissProt: P49766 Mouse
- Unigene: 78781 Human
- Unigene: 15607 Mouse
References for Anti-VEGFB antibody (ab10965)
ab10965 has not yet been referenced specifically in any publications.
Publishing research using ab10965? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"