Overview
- Product nameAnti-VEGFB antibodySee all VEGFB primary antibodies ...
- DescriptionRabbit polyclonal to VEGFB
- Specificityab58461 detects endogenous levels of total VEGFB protein.
- Tested applicationsWB, ELISA, Sandwich ELISA more details
- Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- Immunogen
Synthetic peptide derived from internal sequence of human VEGFB.
- Positive controlExtracts from Jurkat cells
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab58461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
| ELISA | ELISA: 1/20000. |
| Sandwich ELISA | sELISA: Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab76863 as Capture. |
Target
- FunctionGrowth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
- Tissue specificityExpressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
- Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
- Post-translational
modificationsVEGF-B186 is O-glycosylated. - Cellular localizationSecreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
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Database links
- Entrez Gene: 7423 Human
- Entrez Gene: 22340 Mouse
- Entrez Gene: 89811 Rat
- Omim: 601398 Human
- SwissProt: P49765 Human
- SwissProt: P49766 Mouse
- SwissProt: O35485 Rat
- Unigene: 78781 Human
- Unigene: 15607 Mouse
- Unigene: 198550 Rat
see all
Target information above from: UniProt accession
P49765
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- vascular endothelial growth factor B antibodyVascular endothelial growth factor B precursor antibodyVEGF B antibody
- VEGF related factor antibodyVEGF-B antibodyVEGF-related factor antibodyVegfb antibodyVEGFB_HUMAN antibodyVEGFL antibodyVRF antibody
see all
Anti-VEGFB antibody images
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Sandwich ELISA - VEGFB antibody (ab58461)Standard curve for VEGFB (Analyte: ab87052); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse polyclonal to VEGFB (ab76863) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGFB (ab58461) at 0.5µg/ml. -
Western blot - VEGFB antibody (ab58461)All lanes : Anti-VEGFB antibody (ab58461) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunizing peptide
Predicted band size : 22 kDa
Observed band size : 22 kDa
Protocols
References for Anti-VEGFB antibody (ab58461)
This product has been referenced in:
- Guan W et al. Vascular protection by Angiotensin receptor antagonism involves differential VEGF expression in both hemispheres after experimental stroke. PLoS One 6:e24551 (2011). WB ; Rat . Read more (PubMed: 21912702) »
