You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-VEGFB antibody (ab58461)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-VEGFB antibody
See all VEGFB products (6) ...

Description

Rabbit polyclonal to VEGFB

Specificity

ab58461 detects endogenous levels of total VEGFB protein.

Tested applications

WB, ELISA, Sandwich ELISAmore details

Cross reactivity

Reacts with

Rat, Human

Predicted to work with

Mouse

Immunogen

Synthetic peptide derived from internal sequence of human VEGFB.

Positive control

Extracts from Jurkat cells

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> VEGF
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF

  • Sandwich ELISA - VEGFB antibody (ab58461)Sandwich ELISA - VEGFB antibody (ab58461) image (enlarge)

  • Western blot - VEGFB antibody (ab58461)Western blot - VEGFB antibody (ab58461) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab58461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

Tissue specificity

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Sequence similarities

Belongs to the PDGF/VEGF growth factor family.

Post-translational
modifications

VEGF-B186 is O-glycosylated.

Cellular localization

Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Target information above from: UniProt accessionP49765 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Form

There are 2 isoforms produced by alternative splicing.

Alternative names

  • vascular endothelial growth factor B antibody
  • Vascular endothelial growth factor B precursor antibody
  • VEGF B antibody
  • VEGF related factor antibody
  • VEGF-B antibody
  • VEGF-related factor antibody
  • Vegfb antibody
  • VEGFB_HUMAN antibody
  • VEGFL antibody
  • VRF antibody
see all

Database links

see all

Anti-VEGFB antibody images:

  Sandwich ELISA - VEGFB antibody (ab58461)

Sandwich ELISA - VEGFB antibody (ab58461)

Standard curve for VEGFB (Analyte: ab87052); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse polyclonal to VEGFB (ab76863) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGFB (ab58461) at 0.5µg/ml.

  Western blot - VEGFB antibody (ab58461)

Western blot - VEGFB antibody (ab58461)

All lanes : Anti-VEGFB antibody (ab58461) at 1/500 dilution

Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunizing peptide


Predicted band size : 22 kDa
Observed band size : 22 kDa

Protocols

  • Protocol Booklet

    • References for Anti-VEGFB antibody (ab58461)

    This product has been referenced in:

    • Guan Wet al. Vascular protection by Angiotensin receptor antagonism involves differential VEGF expression in both hemispheres after experimental stroke. PLoS One 6:e24551 (2011). WB; Rat.Read more (PubMed: 21912702) »

    See 1 publication for this product

    Publishing research using ab58461? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"