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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
Overview
Product name
Anti-VEGFB antibody
See all VEGFB products (6) ...
Description
Mouse polyclonal to VEGFB
Tested applications
Sandwich ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length human VEGFB protein (NP_003368) with a 26 kDa tag.
Positive control
VEGFB transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> VEGF
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use a concentration of 5 µg/ml Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGFB (ab58461). (For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab58461 as Detection.)
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 22 kDa.
Target
Function
Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
Tissue specificity
Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
Sequence similarities
Belongs to the PDGF/VEGF growth factor family.
Post-translational
modifications
VEGF-B186 is O-glycosylated.
Cellular localization
Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
Target information above from: UniProt accessionP49765
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
There are 2 isoforms produced by alternative splicing.
Alternative names
- Vascular endothelial growth factor B antibody
- Vascular endothelial growth factor B precursor antibody
- VEGF B antibody
see all
Database links
- Entrez Gene: 7423 Human
- Omim: 601398 Human
- SwissProt: P49765 Human
- Unigene: 78781 Human
Anti-VEGFB antibody images:
Sandwich ELISA - VEGFB antibody (ab76863)
Standard curve for VEGFB (Analyte: ab87052); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse polyclonal to VEGFB (ab76863) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGFB (ab58461) at 0.5µg/ml.
Western blot - VEGFB antibody (ab76863)
All lanes : Anti-VEGFB antibody (ab76863) at 5 µg/ml
Lane 1 : VEGFB transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size : 22 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-VEGFB antibody (ab76863)
ab76863 has not yet been referenced specifically in any publications.
Publishing research using ab76863? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"