Overview
- Product nameAnti-VEGFB antibodySee all VEGFB primary antibodies ...
- DescriptionMouse polyclonal to VEGFB
- Tested applicationsSandwich ELISA, WB more details
- Species reactivityReacts with: Human
- Immunogen
Recombinant full length human VEGFB protein (NP_003368) with a 26 kDa tag.
- Positive controlVEGFB transfected 293T cell lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab76863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA: Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGFB (ab58461). For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab58461 as Detection. |
| WB | WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa. |
Target
- FunctionGrowth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
- Tissue specificityExpressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
- Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
- Post-translational
modificationsVEGF-B186 is O-glycosylated. - Cellular localizationSecreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
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Database links
- Entrez Gene: 7423 Human
- Omim: 601398 Human
- SwissProt: P49765 Human
- Unigene: 78781 Human
Target information above from: UniProt accession
P49765
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Vascular endothelial growth factor B antibodyVascular endothelial growth factor B precursor antibodyVEGF B antibody
- VEGF related factor antibodyVEGF-B antibodyVEGF-related factor antibodyVegfb antibodyVEGFB_HUMAN antibodyVEGFL antibodyVRF antibody
see all
Anti-VEGFB antibody images
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Sandwich ELISA - VEGFB antibody (ab76863)Standard curve for VEGFB (Analyte: ab87052); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse polyclonal to VEGFB (ab76863) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGFB (ab58461) at 0.5µg/ml. -
Western blot - VEGFB antibody (ab76863)All lanes : Anti-VEGFB antibody (ab76863) at 5 µg/ml
Lane 1 : VEGFB transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size : 22 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-VEGFB antibody (ab76863)
ab76863 has not yet been referenced specifically in any publications.
