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Anti-VEGFB antibody (ab76863)

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Overview

Product name

Anti-VEGFB antibody
See all VEGFB products (6) ...

Description

Mouse polyclonal to VEGFB

Tested applications

Sandwich ELISA, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant full length human VEGFB protein (NP_003368) with a 26 kDa tag.

Positive control

VEGFB transfected 293T cell lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 1X PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

  • Sandwich ELISA - VEGFB antibody (ab76863)Sandwich ELISA - VEGFB antibody (ab76863) image (enlarge)

  • Western blot - VEGFB antibody (ab76863)Western blot - VEGFB antibody (ab76863) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

Tissue specificity

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Sequence similarities

Belongs to the PDGF/VEGF growth factor family.

Post-translational
modifications

VEGF-B186 is O-glycosylated.

Cellular localization

Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Target information above from: UniProt accessionP49765 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Form

There are 2 isoforms produced by alternative splicing.

Alternative names

  • Vascular endothelial growth factor B antibody
  • Vascular endothelial growth factor B precursor antibody
  • VEGF B antibody
  • VEGF related factor antibody
  • VEGF-B antibody
  • VEGF-related factor antibody
  • Vegfb antibody
  • VEGFB_HUMAN antibody
  • VEGFL antibody
  • VRF antibody
see all

Anti-VEGFB antibody images:

  Sandwich ELISA - VEGFB antibody (ab76863)

Sandwich ELISA - VEGFB antibody (ab76863)

Standard curve for VEGFB (Analyte: ab87052); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse polyclonal to VEGFB (ab76863) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGFB (ab58461) at 0.5µg/ml.

  Western blot - VEGFB antibody (ab76863)

Western blot - VEGFB antibody (ab76863)

All lanes : Anti-VEGFB antibody (ab76863) at 5 µg/ml

Lane 1 : VEGFB transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate

Lysates/proteins at 25 µg per lane.

Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Predicted band size : 22 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)

References for Anti-VEGFB antibody (ab76863)

ab76863 has not yet been referenced specifically in any publications.

Publishing research using ab76863? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"