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Overview

  • Product nameVEGFB protein (Active)See all VEGFB proteins and peptides ...
  • Protein descriptionRecombinant full length Human VEGFB fused to His tag at N-terminal; amino acids 22-207, no signal peptide (207 amino acids), 21.6kDa. Swiss ID P49765.
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notesab87052 was purified using conventional chromatography techniques
  • Biological activityMeasured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml.
    Activity Assay:
    1. Cell line: NIH-3T3 (Mus musculus/Mouse embryonic fibroblast)
    2. Maintenance Condition: RPMI 1640 containing 10% FBS
    3. Assay medium: serum free RPMI 1640
    4. Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul)
    5. Incubation time : 24 hr (after sample treatment)
    6. Concentration range: 10pg/ml - 10ug/ml
    7. Detection method : BrdU assay
    8. Mass spectrometryMALDI-TOF
    9. FormLiquid
    10. Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
    11. Storage bufferPreservative: None
      Constituents: 10% Glycerol, 20mM Tris HCl, pH 7.5
    12. Concentration information loading...
    13. Additional notesEndotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method)
    14. Sequence notesMGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA
    15. Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab87052 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent concentration. Activity assay information can be find in the activity section of this datasheet.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        Vascular endothelial growth factor BVEGF BVEGF related factor
        VEGF-BVEGF-related factorVegfbVEGFB_HUMANVEGFLVRF
      see all
  • FunctionGrowth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
  • Tissue specificityExpressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
  • Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
  • Post-translational
    modifications    VEGF-B186 is O-glycosylated.
  • Cellular localizationSecreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

    • Target information above from: UniProt accession P49765 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    VEGFB protein (Active) images

    References for VEGFB protein (Active) (ab87052)

    ab87052 has not yet been referenced specifically in any publications.

    Publishing research using ab87052 ? Please let us know so that we can cite the reference in this datasheet

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