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Product name
VEGFB protein (His tag)
See all VEGFB products (2) ...
Protein description
Recombinant full length Human VEGFB fused to His tag at N-terminal; amino acids 22-207, no signal peptide (207 amino acids), 21.6kDa. Swiss ID P49765.
Expression host
E. coli
Properties
Purity
> 90% by SDS-PAGE
Purification notes
ab87052 was purified using conventional chromatography techniques
Biological activity
Measured in a cell proliferation assay using NIH-3T3 mouse
embryonic fibroblast. The ED50 for this effect is < 1ng/ml.
Activity Assay:
- Cell line: NIH-3T3 (Mus musculus/Mouse embryonic fibroblast)
- Maintenance Condition: RPMI 1640 containing 10% FBS
- Assay medium: serum free RPMI 1640
- Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul)
- Incubation time : 24 hr (after sample treatment)
- Concentration range: 10pg/ml - 10ug/ml
- Detection method : BrdU assay
Mass spectrometry
MALDI-TOF
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage buffer
Preservative: None
Constituents: 10% Glycerol, 20mM Tris HCl, pH 7.5
Concentration
Concentration information loading...
Additional notes
Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method)
Sequence notes
MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA
Research areas
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> VEGF
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab87052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use at an assay dependent concentration. Activity assay information can be find in the activity section of this datasheet.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
Tissue specificity
Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
Sequence similarities
Belongs to the PDGF/VEGF growth factor family.
Post-translational
modifications
VEGF-B186 is O-glycosylated.
Cellular localization
Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
Target information above from: UniProt accessionP49765
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
VEGFB protein (His tag) images:
Sandwich ELISA - VEGFB protein (His tag) (ab87052)
Standard curve for VEGFB (Analyte: ab87052); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse polyclonal to VEGFB (ab76863) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGFB (ab58461) at 0.5µg/ml.
References for VEGFB protein (His tag) (ab87052)
ab87052 has not yet been referenced specifically in any publications.
Publishing research using ab87052? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"