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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
Overview
Product name
Anti-VEGFC antibody
See all VEGFC products (5) ...
Description
Rabbit polyclonal to VEGFC
Tested applications
IP, WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Highly pure recombinant full length dNdC-VEGF-C protein (Rat).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> VEGF
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use a concentration of 1 - 10 µg/ml.
WB: Use a concentration of 1 µg/ml
ELISA: Use a concentration of 2.5 µg/ml(Allows the detection of 0.5- 1.0 ng/well of recombinant rat VEGF-C.)
IHC-P: Use at an assay dependent dilution.
Target
Function
Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
Tissue specificity
Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.
Sequence similarities
Belongs to the PDGF/VEGF growth factor family.
Post-translational
modifications
Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing an heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
Cellular localization
Secreted.
Target information above from: UniProt accessionP49767
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Flt 4L antibody
- Flt4 ligand antibody
- FLT4 ligand DHM antibody
see all
Database links
- Entrez Gene: 7424 Human
- Entrez Gene: 22341 Mouse
- Entrez Gene: 114111 Rat
- Omim: 601528 Human
- SwissProt: P49767 Human
- SwissProt: P97953 Mouse
- SwissProt: O35757 Rat
- Unigene: 435215 Human
see all
Anti-VEGFC antibody images:
Western blot - VEGFC antibody (ab9546)
Western blot with human and rat VEGF C and VEGF D using the polyclonal anti VEGF C antibody ab9546. Proteins were separated by 15% SDS-PAGE and blotted on to PVDF membranes as described. After saturation, the membrane was incubated for 1 h with ab9546 at 1 µg/ml vascular endothelial growth factor C in TBS containing 20% non-fat milk followed by incubation with a goat-anti-rabbit alkaline phosphatase-conjugated secondary antibody. Lane 1/250ng rat VEGF C; lane 2/250 ng human VEGF C; lane 3/250 ng rat VEGF D; lane4/250 ng human VEGF-D.
Western blot - VEGFC antibody (ab9546)
Anti-VEGFC antibody (ab9546) + Rat Recombinant VEGF-C insect cells
Western Blot was run on a 15% SDS-PAGE gel. Bands between 15kDa and 20kDa represent the different glycoslation forms.
References for Anti-VEGFC antibody (ab9546)
This product has been referenced in:
- Yan Aet al. Mechanisms of lymphatic regeneration after tissue transfer. PLoS One 6:e17201 (2011). IHC-P; Mouse.Read more (PubMed: 21359148) »
- Machnik Aet al. Mononuclear phagocyte system depletion blocks interstitial tonicity-responsive enhancer binding protein/vascular endothelial growth factor C expression and induces salt-sensitive hypertension in rats. Hypertension 55:755-61 (2010). WB, IHC-P; Human.Read more (PubMed: 20142563) »
See all 6 publications for this product
Publishing research using ab9546? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"