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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
Overview
Product name
VEGFC protein (Human)
See all VEGFC products (6) ...
Protein description
Recombinant Human VEGF-C
Expression host
E. coli
Properties
Purity
>90% by SDS-PAGE
Purification notes
Endotoxin level is <0.1 ng per g of VEGF-C
Form
Lyophilised:
reconstitution in 0.1% acetic acid to a concentration of 0.1-1.0 mg/ml.
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Concentration
Concentration information loading...
Research areas
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> VEGF
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> VEGF
Cardiovascular >> Angiogenesis >> Growth Factors >> VEGF >> VEGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE
Application notes
SDS-PAGE: Use at an assay dependent dilution.
Note: Activity of VEGF-C is tested by the stimulation of VEGF-R3 phosphorylation in cells transfected with VEGF-R3. The expected ED50 is > 200 ng/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
Tissue specificity
Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.
Sequence similarities
Belongs to the PDGF/VEGF growth factor family.
Post-translational
modifications
Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing an heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
Cellular localization
Secreted.
Target information above from: UniProt accessionP49767
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for VEGFC protein (Human) (ab51968)
This product has been referenced in:
- Argaw ATet al. IL-1beta regulates blood-brain barrier permeability via reactivation of the hypoxia-angiogenesis program. J Immunol 177:5574-84 (2006).Read more (PubMed: 17015745) »
See 1 publication for this product
Publishing research using ab51968? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"