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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab69989? Please let us know so that we can cite the reference in this datasheet
ab69989 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - VGF antibody (ab69989)
Lane 1 : Anti-VGF antibody (ab69989) at 0.5 µg/ml
Lane 2 : Anti-VGF antibody (ab69989) at 1 µg/ml
Lane 1 : Human brain tissue lysate
Lane 2 : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 67 kDa
Observed band size : 67 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VGF antibody (ab69989)
ab69989 at 5 µg/ml staining VGF in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
Immunocytochemistry/ Immunofluorescence-VGF antibody(ab69989)
ICC/IF image of ab69989 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69989, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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